tRNAmRNA Mimicry by tmRNA and SmpB in Trans-Translation

Abstract EN

Since accurate translation from mRNA to protein is critical to survival, cells have developed translational quality control systems.

Bacterial ribosomes stalled on truncated mRNA are rescued by a system involving tmRNA and SmpB referred to as trans-translation.

Here, we review current understanding of the mechanism of trans-translation.

Based on results obtained by using directed hydroxyl radical probing, we propose a new type of molecular mimicry during trans-translation.

Besides such chemical approaches, biochemical and cryo-EM studies have revealed the structural and functional aspects of multiple stages of trans-translation.

These intensive works provide a basis for studying the dynamics of tmRNA/SmpB in the ribosome.