Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies
Joint Authors
Tanaka, Hiroyuki
Putalun, Waraporn
Shoyama, Yukihiro
Source
Chromatography Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We succeeded in developing the fingerprint of natural product by eastern blotting using monoclonal antibodies.
After developing and separating them on a TLC plate, solasodine glycosides are oxidized by NaIO4 and reacted with a protein to give conjugates which are recognized with anti-solamargine monoclonal antibody (MAb).
Anti-solamargine MAb having wide cross-reactivity can stain and detect all solasodine glycosides by fingerprint.
Different sensitivity between solamargine and solasonine was observed.
The detection limit was 1.6 ng of solasonine.
The hydrolysed products of solamargine were determined by fingerprint of eastern blotting compared to their Rf values depending on the sugar number.
Fingerprint by eastern blotting using anti-ginsenoside Rb1 MAb distinguished the formula containing ginseng prescribed in traditional Chinese medicine.
By double-staining of ginsenosides it is possible to suggest that the staining color shows the pharmacological activity, such as the purple bands indicate ginsenosides having stimulation activity, and the blue color indicated compound like ginsenosides possessed the depression affect for the central nervous system (CNS), respectively.
American Psychological Association (APA)
Tanaka, Hiroyuki& Putalun, Waraporn& Shoyama, Yukihiro. 2012. Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies. Chromatography Research International،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-448117
Modern Language Association (MLA)
Tanaka, Hiroyuki…[et al.]. Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies. Chromatography Research International No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-448117
American Medical Association (AMA)
Tanaka, Hiroyuki& Putalun, Waraporn& Shoyama, Yukihiro. Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies. Chromatography Research International. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-448117
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448117