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Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes
Joint Authors
Ziegler, Cornelia
Schmitteckert, Stefanie
Kartes, Liane
Rolletschek, Alexandra
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Transcription factor Lbx1 is known to play a role in the migration of muscle progenitor cells in limb buds and also in neuronal determination processes.
In addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated.
Here, we used mouse embryonic stem (ES) cells which have the capacity to develop into cells of all three primary germ layers.
During in vitro differentiation, ES cells recapitulate cellular developmental processes and gene expression patterns of early embryogenesis.
Transcript analysis revealed a significant upregulation of Lbx1 at the progenitor cell stage.
Immunofluorescence staining confirmed the expression of Lbx1 in skeletal muscle cell progenitors and GABAergic neurons.
To verify the presence of Lbx1 in cardiac cells, triple immunocytochemistry of ES cell-derived cardiomyocytes and a quantification assay were performed at different developmental stages.
Colabeling of Lbx1 and cardiac specific markers troponin T, α-actinin, GATA4, and Nkx2.5 suggested a potential role in early myocardial development.
American Psychological Association (APA)
Schmitteckert, Stefanie& Ziegler, Cornelia& Kartes, Liane& Rolletschek, Alexandra. 2011. Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes. Stem Cells International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-448141
Modern Language Association (MLA)
Schmitteckert, Stefanie…[et al.]. Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes. Stem Cells International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-448141
American Medical Association (AMA)
Schmitteckert, Stefanie& Ziegler, Cornelia& Kartes, Liane& Rolletschek, Alexandra. Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-448141
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448141