Improved Label-Free LC-MS Analysis by Wavelet-Based Noise Rejection
Joint Authors
Cerutti, Sergio
Benevento, Marco
Roda, Aldo
Cappadona, Salvatore
Pattini, Linda
Levander, Fredrik
Nanni, Paolo
Versura, Piera
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Label-free LC-MS analysis allows determining the differential expression level of proteins in multiple samples, without the use of stable isotopes.
This technique is based on the direct comparison of multiple runs, obtained by continuous detection in MS mode.
Only differentially expressed peptides are selected for further fragmentation, thus avoiding the bias toward abundant peptides typical of data-dependent tandem MS.
The computational framework includes detection, alignment, normalization and matching of peaks across multiple sets, and several software packages are available to address these processing steps.
Yet, more care should be taken to improve the quality of the LC-MS maps entering the pipeline, as this parameter severely affects the results of all downstream analyses.
In this paper we show how the inclusion of a preprocessing step of background subtraction in a common laboratory pipeline can lead to an enhanced inclusion list of peptides selected for fragmentation and consequently to better protein identification.
American Psychological Association (APA)
Cappadona, Salvatore& Nanni, Paolo& Benevento, Marco& Levander, Fredrik& Versura, Piera& Roda, Aldo…[et al.]. 2010. Improved Label-Free LC-MS Analysis by Wavelet-Based Noise Rejection. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-448179
Modern Language Association (MLA)
Cappadona, Salvatore…[et al.]. Improved Label-Free LC-MS Analysis by Wavelet-Based Noise Rejection. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-448179
American Medical Association (AMA)
Cappadona, Salvatore& Nanni, Paolo& Benevento, Marco& Levander, Fredrik& Versura, Piera& Roda, Aldo…[et al.]. Improved Label-Free LC-MS Analysis by Wavelet-Based Noise Rejection. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-448179
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448179