![](/images/graphics-bg.png)
Synthetic Pre-miRNA-Based shRNA as Potent RNAi Triggers
Joint Authors
Shimizu, Kazuharu
Terasawa, Kazuya
Tsujimoto, Gozoh
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
RNA interference (RNAi) is a powerful tool for studying gene function owing to the ease with which it can selectively silence genes of interest, and it has also attracted attention because of its potential for therapeutic applications.
Chemically synthesized small interfering RNAs (siRNAs) and DNA vector-based short hairpin RNAs (shRNAs) are now widely used as RNAi triggers.
In contrast to expressed shRNAs, the use of synthetic shRNAs is limited.
Here we designed shRNAs modeled on a precursor microRNA (pre-miRNA) and evaluated their biological activity.
We demonstrated that chemically synthetic pre-miRNA-based shRNAs have more potent RNAi activity than their corresponding siRNAs and found that their antisense strands are more efficiently incorporated into the RNA-induced silencing complex.
Although greater off-target effects and interferon responses were induced by shRNAs than by their corresponding siRNAs, these effects could be overcome by simply using a lower concentration or by optimizing and chemically modifying shRNAs similar to synthetic siRNAs.
These are challenges for the future.
American Psychological Association (APA)
Terasawa, Kazuya& Shimizu, Kazuharu& Tsujimoto, Gozoh. 2011. Synthetic Pre-miRNA-Based shRNA as Potent RNAi Triggers. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-448183
Modern Language Association (MLA)
Terasawa, Kazuya…[et al.]. Synthetic Pre-miRNA-Based shRNA as Potent RNAi Triggers. Journal of Nucleic Acids No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-448183
American Medical Association (AMA)
Terasawa, Kazuya& Shimizu, Kazuharu& Tsujimoto, Gozoh. Synthetic Pre-miRNA-Based shRNA as Potent RNAi Triggers. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-448183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448183