Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls
Joint Authors
Martins, Marcia V.
Bekele, Yonas
Fantahun, Yonas
van der Ploeg-van Schip, Jolien J.
Pessolani, Maria C. V.
Bobosha, Kidist
Esquenazi, Danuza A.
Ottenhoff, Tom H. M.
Aseffa, Abraham
Pereira, Geraldo M. B.
Gismondi, Ronaldo C.
Guimarães, Marjorie M.
Franken, Kees L. M. C.
Geluk, Annemieke
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The stable incidence of new leprosy cases suggests that transmission of infection continues despite worldwide implementation of MDT.
Thus, specific tools are needed to diagnose early stage Mycobacterium leprae infection, the likely sources of transmission.
M.
leprae antigens that induce T-cell responses in M.
leprae exposed and/or infected individuals thus are major targets for new diagnostic tools.
Previously, we showed that ML1601c was immunogenic in patients and healthy household contacts (HHC).
However, some endemic controls (EC) also recognized this protein.
To improve the diagnostic potential, IFN-γ responses to ML1601c peptides were assessed using PBMC from Brazilian leprosy patients and EC.
Five ML1601c peptides only induced IFN-γ in patients and HHC.
Moreover, 24-hour whole-blood assay (WBA), two ML1601c peptides could assess the level of M.
leprae exposure in Ethiopian EC.
Beside IFN-γ, also IP-10, IL-6, IL-1β, TNF-α, and MCP-1 were increased in EC from areas with high leprosy prevalence in response to these ML1601c peptides.
Thus, ML1601c peptides may be useful for differentiating M.
leprae exposed or infected individuals and can also be used to indicate the magnitude of M.
leprae transmission even in the context of various HLA alleles as present in these different genetic backgrounds.
American Psychological Association (APA)
Bobosha, Kidist& van der Ploeg-van Schip, Jolien J.& Esquenazi, Danuza A.& Guimarães, Marjorie M.& Martins, Marcia V.& Bekele, Yonas…[et al.]. 2012. Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls. Journal of Tropical Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-448218
Modern Language Association (MLA)
Bobosha, Kidist…[et al.]. Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls. Journal of Tropical Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-448218
American Medical Association (AMA)
Bobosha, Kidist& van der Ploeg-van Schip, Jolien J.& Esquenazi, Danuza A.& Guimarães, Marjorie M.& Martins, Marcia V.& Bekele, Yonas…[et al.]. Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls. Journal of Tropical Medicine. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-448218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448218