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Effect of One Year of Cryopreservation on the Activity of Lysosomal Hydrolases from EBV-Transformed Lymphocytes
Joint Authors
Michelin-Tireli, K.
Coelho, J. C.
Camelier, M. V.
de Mello, A. S.
Mendes, F. B.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Background.
The Epstein-Barr virus (EBV) was used as an agent of B lymphocyte proliferation for subsequent diagnosis of lysosomal storage disease.
Due to the constant handling of long-preserved samples in our cell bank, we decided to observe the behavior and then compare cultured and frozen samples for at least one year's cryopreservation.
Methods.
Twenty-five samples from healthy individuals were used to assess the possible changes in activity of enzymes β-galactosidase, β-glucosidase, α-iduronidase, α-galactosidase, and α-glucosidase.
Transmission electron microscopy was used to confirm cell transformation of B lymphocytes into EBV-infected cells, generating lymphoblastoid cell lines.
Results.
Transmission electron microscopy findings confirmed previous reports in the literature that is, significant and evident morphological changes in the nucleus occur after day 12 and the consequent cell transformation into EBV-infected cells.
After thawing and subsequent treatment with the five enzymes utilized, we observed no significant changes in samples cryopreserved for more than one year, as compared to samples cultured for 12 days.
American Psychological Association (APA)
de Mello, A. S.& Mendes, F. B.& Michelin-Tireli, K.& Camelier, M. V.& Coelho, J. C.. 2011. Effect of One Year of Cryopreservation on the Activity of Lysosomal Hydrolases from EBV-Transformed Lymphocytes. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-448272
Modern Language Association (MLA)
de Mello, A. S.…[et al.]. Effect of One Year of Cryopreservation on the Activity of Lysosomal Hydrolases from EBV-Transformed Lymphocytes. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-448272
American Medical Association (AMA)
de Mello, A. S.& Mendes, F. B.& Michelin-Tireli, K.& Camelier, M. V.& Coelho, J. C.. Effect of One Year of Cryopreservation on the Activity of Lysosomal Hydrolases from EBV-Transformed Lymphocytes. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-448272
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448272