Microemulsion Liquid Chromatographic Method for Simultaneous Determination of Simvastatin and Ezetimibe in Their Combined Dosage Forms
Joint Authors
Hammouda, Mohammed E. A.
El-Sherbiny, Dina T.
El-Ashry, Saadia M.
Abu El-Enin, Mohamed A.
El-Wasseef, Dalia R.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A rapid HPLC procedure using a microemulsion as an eluent was developed and validated for analytical quality control of antihyperlipidemic mixture containing simvastatin (SIM) and ezetimibe (EZT) in their pharmaceutical preparations.
The separation was performed on a column packed with cyano bonded stationary phase adopting UV detection at 238 nm using a flow rate of 1 mL/min.
The optimized microemulsion mobile phase consisted of 0.2 M sodium dodecyl sulphate, 1% octanol, 10% n-propanol, and 0.3% triethylamine in 0.02 M phosphoric acid at pH 5.0.
The developed method was validated in terms of specificity, linearity, lower limit of quantification (LOQ), lower limit of detection (LOD), precision, and accuracy.
The proposed method is rapid (8.5 min), reproducible (RSD < 2.0%) and achieves satisfactory resolution between SIM and EZT (resolution factor = 2.57).
The mean recoveries of the analytes in pharmaceutical preparations were in agreement with those obtained from a reference method, as revealed by statistical analysis of the obtained results using Student’s t-test and the variance ratio F-test.
American Psychological Association (APA)
Hammouda, Mohammed E. A.& Abu El-Enin, Mohamed A.& El-Sherbiny, Dina T.& El-Wasseef, Dalia R.& El-Ashry, Saadia M.. 2013. Microemulsion Liquid Chromatographic Method for Simultaneous Determination of Simvastatin and Ezetimibe in Their Combined Dosage Forms. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-448301
Modern Language Association (MLA)
Hammouda, Mohammed E. A.…[et al.]. Microemulsion Liquid Chromatographic Method for Simultaneous Determination of Simvastatin and Ezetimibe in Their Combined Dosage Forms. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-448301
American Medical Association (AMA)
Hammouda, Mohammed E. A.& Abu El-Enin, Mohamed A.& El-Sherbiny, Dina T.& El-Wasseef, Dalia R.& El-Ashry, Saadia M.. Microemulsion Liquid Chromatographic Method for Simultaneous Determination of Simvastatin and Ezetimibe in Their Combined Dosage Forms. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-448301
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448301