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Phytochemical Activation of Nrf2 Protects Human Coronary Artery Endothelial Cells against an Oxidative Challenge
Joint Authors
Hamilton, Karyn L.
Reuland, Danielle J.
McCord, Joe M.
Miller, Benjamin F.
Donovan, Elise L.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Activation of NF-E2-related factor 2 (Nrf2) is a potential therapeutic intervention against endothelial cell oxidative stress and associated vascular disease.
We hypothesized that treatment with the phytochemicals in the patented dietary supplement Protandim would induce Nrf2 nuclear localization and phase II antioxidant enzyme protein in human coronary artery endothelial cells (HCAECs), protecting against an oxidant challenge in an Nrf2- dependent manner.
Protandim treatment induced Nrf2 nuclear localization, and HO-1 (778% of control ± 82.25 P<0.01), SOD1 (125.9% of control ± 6.05 P<0.01), NQO1 (126% of control ± 6.5 P<0.01), and GR (119.5% of control ± 7.00 P<0.05) protein expression in HCAEC.
Treatment of HCAEC with H2O2 induced apoptosis in 34% of cells while pretreatment with Protandim resulted in only 6% apoptotic cells (P<0.01).
Nrf2 silencing significantly decreased the Protandim-induced increase in HO-1 protein (P<0.01).
Nrf2 silencing also significantly decreased the protection afforded by Protandim against H2O2- induced apoptosis (P<0.01 compared to no RNA, and P<0.05 compared to control RNA).
These results show that Protandim induces Nrf2 nuclear localization and antioxidant enzyme expression, and protection of HCAEC from an oxidative challenge is Nrf2 dependent.
American Psychological Association (APA)
Donovan, Elise L.& McCord, Joe M.& Reuland, Danielle J.& Miller, Benjamin F.& Hamilton, Karyn L.. 2012. Phytochemical Activation of Nrf2 Protects Human Coronary Artery Endothelial Cells against an Oxidative Challenge. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-448310
Modern Language Association (MLA)
Donovan, Elise L.…[et al.]. Phytochemical Activation of Nrf2 Protects Human Coronary Artery Endothelial Cells against an Oxidative Challenge. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-448310
American Medical Association (AMA)
Donovan, Elise L.& McCord, Joe M.& Reuland, Danielle J.& Miller, Benjamin F.& Hamilton, Karyn L.. Phytochemical Activation of Nrf2 Protects Human Coronary Artery Endothelial Cells against an Oxidative Challenge. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-448310
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448310