Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus
Joint Authors
Negron, Leonardo
Patchett, Mark L.
Parker, Emily J.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Dehydroquinate synthase (DHQS) catalyses the second step of the shikimate pathway to aromatic compounds.
DHQS from the archaeal hyperthermophile Pyrococcus furiosus was insoluble when expressed in Escherichia coli but was partially solubilised when KCl was included in the cell lysis buffer.
A purification procedure was developed, involving lysis by sonication at 30∘C followed by a heat treatment at 70∘C and anion exchange chromatography.
Purified recombinant P.
furiosus DHQS is a dimer with a subunit Mr of 37,397 (determined by electrospray ionisation mass spectrometry) and is active over broad pH and temperature ranges.
The kinetic parameters are KM (3-deoxy-D-arabino-heptulosonate 7-phosphate) 3.7 μM and k cat 3.0 sec-1 at 60∘C and pH 6.8.
EDTA inactivates the enzyme, and enzyme activity is restored by several divalent metal ions including (in order of decreasing effectiveness) Cd2+, Co2+, Zn2+, and Mn2+.
High activity of a DHQS in the presence of Cd2+ has not been reported for enzymes from other sources, and may be related to the bioavailability of Cd2+ for P.
furiosus.
This study is the first biochemical characterisation of a DHQS from a thermophilic source.
Furthermore, the characterisation of this hyperthermophilic enzyme was carried out at elevated temperatures using an enzyme-coupled assay.
American Psychological Association (APA)
Negron, Leonardo& Patchett, Mark L.& Parker, Emily J.. 2011. Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus. Enzyme Research،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-448416
Modern Language Association (MLA)
Negron, Leonardo…[et al.]. Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus. Enzyme Research No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-448416
American Medical Association (AMA)
Negron, Leonardo& Patchett, Mark L.& Parker, Emily J.. Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-448416
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448416