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Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51
Joint Authors
Denizci, Aziz Akin
Kuzu, Secil Berna
Güvenmez, Hatice Korkmaz
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Information Technology and Computer Science
Abstract EN
This paper reports the isolation and identification of chitinase-producing Bacillus from chitin-containing wastes, production of a thermostable and alkaline chitinasese, and enzyme characterization.
Bacillus thuringiensis subsp.
kurstaki HBK-51 was isolated from soil and was identified.
Chitinase was obtained from supernatant of B.
thuringiensis HBK-51 strain and showed its optimum activity at 110°C and at pH 9.0.
Following 3 hours of incubation period, the enzyme showed a high level of activity at 110°C (96% remaining activity) and between pH 9.0 and 12.0 (98% remaining activity).
Considering these characteristics, the enzyme was described as hyperthermophile-thermostable and highly alkaline.
Two bands of the enzyme weighing 50 and 125 kDa were obtained following 12% SDS-PAGE analyses.
Among the metal ions and chemicals used, Ni2+ (32%), K+ (44%), and Cu2+ (56%) increased the enzyme activity while EDTA (7%), SDS (7%), Hg2+ (11%), and ethyl-acetimidate (20%) decreased the activity of the enzyme.
Bacillus thuringiensis subsp.
kurstaki HBK-51 is an important strain which can be used in several biotechnological applications as a chitinase producer.
American Psychological Association (APA)
Kuzu, Secil Berna& Güvenmez, Hatice Korkmaz& Denizci, Aziz Akin. 2012. Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-448488
Modern Language Association (MLA)
Kuzu, Secil Berna…[et al.]. Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51. Biotechnology Research International No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-448488
American Medical Association (AMA)
Kuzu, Secil Berna& Güvenmez, Hatice Korkmaz& Denizci, Aziz Akin. Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-448488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448488