Liquid ChromatographyTandem Mass Spectrometry Method for Quantitation of Cremophor EL and Its Applications
Joint Authors
Vijaya Bhaskar, V.
Middha, Anil
Source
International Journal of Analytical Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A rapid sensitive and selective MRM based method for the determination of Cremophor EL (CrEL) in rat plasma was developed using liquid chromatography/tandem mass spectrometry (LC-MS/MS).
CrEL and polypropylene glycol (internal standard) were extracted from rat plasma with acetonitrile and analysed on C18 column (XBridge, 50 × 4.6 mm, 3.5 μm).
The most abundant molecular ions corresponding to PEG oligomers at m/z 828, 872, 916 and 960 with daughter ion at m/z 89 were selected for multiple reaction monitoring (MRM) in electrospray mode of ionisation.
Plasma concentrations of CrEL were quantified after administration through oral and intravenous routes in male sprague dawley rats at a dose of 0.26 g/kg.
The standard curve was linear (0.9972) over the concentration range of 1.00 to 200 μg/mL.
The lower limit of quantitation for CrEL was 1.00 μg/mL using 50 μL plasma.
The coefficient of variation and relative error for inter and intra assay at three QC levels were 0.69 to 9.21 and −7.60 to 4.74 respectively.
A novel proposal was conveyed to the scientific community, where formulation excipient can be analysed as qualifier in the analysis of NCEs to address the spiky plasma concentration profiles.
American Psychological Association (APA)
Vijaya Bhaskar, V.& Middha, Anil. 2013. Liquid ChromatographyTandem Mass Spectrometry Method for Quantitation of Cremophor EL and Its Applications. International Journal of Analytical Chemistry،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-448490
Modern Language Association (MLA)
Vijaya Bhaskar, V.& Middha, Anil. Liquid ChromatographyTandem Mass Spectrometry Method for Quantitation of Cremophor EL and Its Applications. International Journal of Analytical Chemistry No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-448490
American Medical Association (AMA)
Vijaya Bhaskar, V.& Middha, Anil. Liquid ChromatographyTandem Mass Spectrometry Method for Quantitation of Cremophor EL and Its Applications. International Journal of Analytical Chemistry. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-448490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448490