Aquaporin-1 Protein Levels Elevated in Fresh Urine of Renal Cell Carcinoma Patients : Potential Use for Screening and Classification of Incidental Renal Lesions
Joint Authors
Petros, John A.
Lian, Fei
Pattaras, John G.
Sreedharan, Shilpa
Arnold, Rebecca S.
Ogan, Kenneth
Master, Viraj A.
Roberts, David L.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-06
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Introduction and Objectives.
There are over 65,000 new cases of renal cell carcinoma (RCC) each year, yet there is no effective clinical screening test for RCC.
A single report claimed no overlap between urine levels of aquaporin-1 (AQP1) in patients with and without RCC (Mayo Clin Proc.
85:413, 2010).
Here, we used archived and fresh RCC patient urine to validate this report.
Methods.
Archived RCC, fresh prenephrectomy RCC, and non-RCC negative control urines were processed for Western blot analysis.
Urinary creatinine concentrations were quantified by the Jaffe reaction (Nephron 16:31, 1976).
Precipitated protein was dissolved in 1x SDS for a final concentration of 2 μg/µL creatinine.
Results.
Negative control and archived RCC patient urine failed to show any AQP1 protein by Western blot analysis.
Fresh RCC patient urine is robustly positive for AQP1.
There was no signal overlap between fresh RCC and negative control, making differentiation straightforward.
Conclusions.
Our data confirms that fresh urine of patients with RCC contains easily detectable AQP1 protein.
However, archival specimens showed an absence of detectable AQP1 indistinguishable from negative control.
These findings suggest that a clinically applicable diagnostic test for AQP1 in fresh urine may be useful for detecting RCC.
American Psychological Association (APA)
Sreedharan, Shilpa& Petros, John A.& Master, Viraj A.& Ogan, Kenneth& Pattaras, John G.& Roberts, David L.…[et al.]. 2014. Aquaporin-1 Protein Levels Elevated in Fresh Urine of Renal Cell Carcinoma Patients : Potential Use for Screening and Classification of Incidental Renal Lesions. Disease Markers،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-448496
Modern Language Association (MLA)
Sreedharan, Shilpa…[et al.]. Aquaporin-1 Protein Levels Elevated in Fresh Urine of Renal Cell Carcinoma Patients : Potential Use for Screening and Classification of Incidental Renal Lesions. Disease Markers No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-448496
American Medical Association (AMA)
Sreedharan, Shilpa& Petros, John A.& Master, Viraj A.& Ogan, Kenneth& Pattaras, John G.& Roberts, David L.…[et al.]. Aquaporin-1 Protein Levels Elevated in Fresh Urine of Renal Cell Carcinoma Patients : Potential Use for Screening and Classification of Incidental Renal Lesions. Disease Markers. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-448496
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448496