![](/images/graphics-bg.png)
The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance
Joint Authors
Komers, Radko
Dankova, Helena
Sticova, Eva
Zdychova, Jana
Cahova, Monika
Kazdova, Ludmila
Palenickova, Eliska
Papackova, Zuzana
Source
Biochemistry Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-23
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Topics
Abstract EN
We tested the hypothesis that TAG accumulation in the liver induced by short-term high-fat diet (HFD) in rats leads to the dysregulation of endogenous TAG degradation by lysosomal lipase (LIPA) via lysosomal pathway and is causally linked with the onset of hepatic insulin resistance.
We found that LIPA could be translocated between qualitatively different depots (light and dense lysosomes).
In contrast to dense lysosomal fraction, LIPA associated with light lysosomes exhibits high activity on both intracellular TAG and exogenous substrate and prandial- or diet-dependent regulation.
On standard diet, LIPA activity was upregulated in fasted and downregulated in fed animals.
In the HFD group, we demonstrated an increased TAG content, elevated LIPA activity, enhanced production of diacylglycerol, and the abolishment of prandial-dependent LIPA regulation in light lysosomal fraction.
The impairment of insulin signalling and increased activation of PKCε was found in liver of HFD-fed animals.
Lipolysis of intracellular TAG, mediated by LIPA, is increased in steatosis probably due to the enhanced formation of phagolysosomes.
Consequent overproduction of diacylglycerol may represent the causal link between HFD-induced hepatic TAG accumulation and hepatic insulin resistance via PKCε activation.
American Psychological Association (APA)
Cahova, Monika& Dankova, Helena& Palenickova, Eliska& Papackova, Zuzana& Komers, Radko& Zdychova, Jana…[et al.]. 2011. The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance. Biochemistry Research International،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-448503
Modern Language Association (MLA)
Cahova, Monika…[et al.]. The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance. Biochemistry Research International No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-448503
American Medical Association (AMA)
Cahova, Monika& Dankova, Helena& Palenickova, Eliska& Papackova, Zuzana& Komers, Radko& Zdychova, Jana…[et al.]. The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance. Biochemistry Research International. 2011. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-448503
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448503