Ion Suppression Study for Tetracyclines in Feed
Joint Authors
van Holthoon, Frederique
Zuidema, Tina
Chico, Joaquim
Source
Chromatography Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Ion suppression in analysis of tetracyclines in feed was studied.
The conventional analysis consists of a liquid extraction followed by a clean-up step using solid phase extraction (SPE) technique and analysis of the tetracyclines by liquid chromatography and mass spectrometric detection.
Various strategies for extraction and cleanup were tested in the present work, and the effectiveness to decrease the ion suppression on the MS/MS signals was evaluated.
Four sample treatment methods were tested with five different feed samples.
Extraction solvents tested were McIlvaine buffer and a mixture of McIlvaine buffer dichloromethane (3 : 1).
SPE cartridges for cleanup were Oasis HLB, Oasis MCX, and Oasis MAX.
The effectiveness of the methods was evaluated in terms of decreasing the ion suppression effect but also of decreasing the variability of ion suppression between samples.
The method that provided the most satisfactory results involved a clean-up step based on SPE using mixed-mode cation exchange cartridges (Oasis MCX).
American Psychological Association (APA)
Chico, Joaquim& van Holthoon, Frederique& Zuidema, Tina. 2012. Ion Suppression Study for Tetracyclines in Feed. Chromatography Research International،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-448517
Modern Language Association (MLA)
Chico, Joaquim…[et al.]. Ion Suppression Study for Tetracyclines in Feed. Chromatography Research International No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-448517
American Medical Association (AMA)
Chico, Joaquim& van Holthoon, Frederique& Zuidema, Tina. Ion Suppression Study for Tetracyclines in Feed. Chromatography Research International. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-448517
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448517