An Evaluation on Transfection Efficiency of pHRE-Egr1-EGFP in Hepatocellular Carcinoma Cells Bel-7402 Mediated by PEI-MZF-NPs
Joint Authors
Xiao, Li
Zhang, Dongsheng
Guo, Ting
Zhang, Lixin
Lin, Mei
Ye, Jun
Huang, Junxing
Zhang, Jia
Wang, Li
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Civil Engineering
Abstract EN
To improve transfection and expression efficiency of target gene, especially under cancer anoxic microenvironment, we have developed pHRE-Egr1-EGFP/PEI-MZF-NPs nanosystem, in which pHRE-Egr1-EGFP, eukaryotic gene expression plasmid, is constructed by combining radiation promoter Egr1 with anoxia induction components (HRE), forming anoxic radiation double sensitive HRE/Egr1 promoter to activate reporter gene EGFP expression.
MZF-NPs (Mn0.5 Zn0.5 Fe2O4 magnetic nanoparticles), obtained by coprecipitation method, are coated with cation poly(ethylenimine) (PEI).
We transferred pHRE-Egr1-EGFP into hepatocellular carcinoma Bel-7402 cells, using PEI-MZF-NPs as the carrier and tested some relevant efficacy.
The results show that PEI-MZF-NPs have good DNA-binding ability, protection ability, release ability, little toxicity, and high transfection efficiency, obviously superior to those of the liposome method and electricity perforation method.
Moreover, the expression level of EGFP gene induced by anoxia and radiation was significantly higher than that of single radiation activation.
It is therefore concluded that HRE/Egr1 can induce and improve target gene expression efficiency in cancer anoxic microenvironment, and that PEI-MZF-NPs can be used as a novel nonviral gene vector which offers a viable approach to the mediated radiation gene therapy of cancer.
American Psychological Association (APA)
Lin, Mei& Zhang, Dongsheng& Huang, Junxing& Zhang, Jia& Wang, Li& Guo, Ting…[et al.]. 2011. An Evaluation on Transfection Efficiency of pHRE-Egr1-EGFP in Hepatocellular Carcinoma Cells Bel-7402 Mediated by PEI-MZF-NPs. Journal of Nanomaterials،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-448533
Modern Language Association (MLA)
Lin, Mei…[et al.]. An Evaluation on Transfection Efficiency of pHRE-Egr1-EGFP in Hepatocellular Carcinoma Cells Bel-7402 Mediated by PEI-MZF-NPs. Journal of Nanomaterials No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-448533
American Medical Association (AMA)
Lin, Mei& Zhang, Dongsheng& Huang, Junxing& Zhang, Jia& Wang, Li& Guo, Ting…[et al.]. An Evaluation on Transfection Efficiency of pHRE-Egr1-EGFP in Hepatocellular Carcinoma Cells Bel-7402 Mediated by PEI-MZF-NPs. Journal of Nanomaterials. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-448533
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448533