![](/images/graphics-bg.png)
Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma
Joint Authors
El-Koussi, Wesam M.
Atia, Noha N.
El-Shabouri, Salwa R.
Mahmoud, Ashraf M.
Source
International Journal of Analytical Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Two new, sensitive, and selective spectrofluorometric methods were developed for the determination of gemifloxacin mesylate (GFX) in tablets and spiked human plasma.
Method A was based on measurement of the enhanced fluorescence spectral behaviour of GFX in a sodium dodecyl sulphate (SDS) micellar system.
In aqueous solution of acetate buffer pH 5.5, the fluorescence intensity of GFX was greatly enhanced about tenfold in the presence of SDS.
The fluorescence intensity was measured at 402 nm after excitation at 274 nm.
Method B was based on Hantzsch condensation reaction between the primary amino group of GFX with acetylacetone and formaldehyde in acetate buffer of pH 3.5 yielding a highly yellow fluorescent derivative.
The reaction of GFX with acetylacetone-formaldehyde system solution resulted in bathochromic shift of both emission (476 nm) and excitation (420 nm) wavelengths.
The fluorescence intensity was directly proportional to the concentration over the range 10–1000 ng/ml and 100–2000 ng/ml for method A and B, respectively.
The proposed methods were applied successfully for determination of GFX in its tablets and spiked plasma.
Therefore, these methods can be considered of real interest for reliable and practical quality control analysis of GFX.
American Psychological Association (APA)
Atia, Noha N.& Mahmoud, Ashraf M.& El-Shabouri, Salwa R.& El-Koussi, Wesam M.. 2013. Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma. International Journal of Analytical Chemistry،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-448641
Modern Language Association (MLA)
Atia, Noha N.…[et al.]. Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma. International Journal of Analytical Chemistry No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-448641
American Medical Association (AMA)
Atia, Noha N.& Mahmoud, Ashraf M.& El-Shabouri, Salwa R.& El-Koussi, Wesam M.. Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma. International Journal of Analytical Chemistry. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-448641
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448641