Investigation on the Competition Interaction of Synthetic Food Colorants and Ciprofloxacin Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy
Joint Authors
Yang, Chao
Lü, Yunkai
Wang, Jing
Xue, Chunli
Liu, Baosheng
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The effects of synthetic food colorants like tartrazine (TTZ), sunset yellow (SY), and erythrosine (ETS) on the binding reaction between ciprofloxacin hydrochloride (CPFX) and bovine serum albumin (BSA) were investigated by fluorescence spectroscopy in the aqueous solution of pH = 7.40.
Results showed that CPFX caused the fluorescence quenching of BSA through a static quenching procedure and the primary binding site was located at subdomain IIA of BSA (site I).
According to the calculated thermodynamic parameters, it confirmed that CPFX bound to BSA by electrostatic interaction.
In addition, the colorants affected the formation of BSA-CPFX complex.
This resulted in an increase of the free, biological active fraction of CPFX.
The binding distance of BSA-CPFX systems was evaluated according to Förster's theory.
Results suggested that the binding distance were increased in the presence of synthetic food colorants.
American Psychological Association (APA)
Liu, Baosheng& Yang, Chao& Wang, Jing& Xue, Chunli& Lü, Yunkai. 2011. Investigation on the Competition Interaction of Synthetic Food Colorants and Ciprofloxacin Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy. Journal of Thermodynamics،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-448670
Modern Language Association (MLA)
Liu, Baosheng…[et al.]. Investigation on the Competition Interaction of Synthetic Food Colorants and Ciprofloxacin Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy. Journal of Thermodynamics No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-448670
American Medical Association (AMA)
Liu, Baosheng& Yang, Chao& Wang, Jing& Xue, Chunli& Lü, Yunkai. Investigation on the Competition Interaction of Synthetic Food Colorants and Ciprofloxacin Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy. Journal of Thermodynamics. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-448670
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448670