Statistical Analysis of Metal Chelating Activity of Centella asiatica and Erythroxylum cuneatum Using Response Surface Methodology
Joint Authors
Mohd Salim, R. J.
Sued, A. S.
Jauri, M. H.
Amid, A.
Adenan, M. I.
Source
Biotechnology Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-27
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Information Technology and Computer Science
Abstract EN
The purpose of the study is to evaluate the relationship between the extraction parameters and the metal chelating activity of Centella asiatica (CA) and Erythroxylum cuneatum (EC).
The response surface methodology was used to optimize the extraction parameters of methanolic extract of CA and EC with respect to the metal chelating activity.
For CA, Run 17 gave optimum chelating activity with IC50 = 0.93 mg/mL at an extraction temperature of 25°C, speed of agitation at 200 rpm, ratio of plant material to solvent at 1 g : 45 mL and extraction time at 1.5 hour.
As for EC, Run 13 with 60°C, 200 rpm, 1 g : 35 mL and 1 hour had metal chelating activity at IC50 = 0.3817 mg/mL.
Both optimized extracts were further partitioned using a solvent system to evaluate the fraction responsible for the chelating activity of the plants.
The hexane fraction of CA showed potential activity with chelating activity at IC50 = 0.090 and the ethyl acetate fraction of EC had IC50 = 0.120 mg/mL.
The study showed that the response surface methodology helped to reduce the extraction time, temperature and agitation and subsequently improve the chelating activity of the plants in comparison to the conventional method.
American Psychological Association (APA)
Mohd Salim, R. J.& Adenan, M. I.& Amid, A.& Jauri, M. H.& Sued, A. S.. 2013. Statistical Analysis of Metal Chelating Activity of Centella asiatica and Erythroxylum cuneatum Using Response Surface Methodology. Biotechnology Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-448694
Modern Language Association (MLA)
Mohd Salim, R. J.…[et al.]. Statistical Analysis of Metal Chelating Activity of Centella asiatica and Erythroxylum cuneatum Using Response Surface Methodology. Biotechnology Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-448694
American Medical Association (AMA)
Mohd Salim, R. J.& Adenan, M. I.& Amid, A.& Jauri, M. H.& Sued, A. S.. Statistical Analysis of Metal Chelating Activity of Centella asiatica and Erythroxylum cuneatum Using Response Surface Methodology. Biotechnology Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-448694
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448694