Expression of Chemoresistance-Related Genes and Heat Shock Protein 72 in Hyperthermic Isolated Limb Perfusion of Malignant Melanoma : An Experimental Study
Joint Authors
Meyer, T.
Göhl, J.
Pelz, J. O.
Hohenberger, Werner
Knorr, C.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Hyperthermic isolated limb perfusion (HILP) is considered an established treatment for multiple locoregional intransit metastases in malignant melanoma of the extremities.
Various mechanisms such as the expression of chemoresistance genes and heat shock proteins by the tumor may be responsible for varying response rates and locoregional recurrences of the treatment.
The aim of the experimental animal study was to investigate the direct impact of HILP on such mechanisms of resistance.
Tissue temperature, administration of the cytostatic drug, and duration of perfusion were varied.
Expression of the chemoresistance genes mdr1, mrp1, mrp2, and lrp and of heat shock protein 72 (HSP72) in the tumor tissue was analysed using RT-PCR and western blot analysis.
The untreated SK-MEL-3 tumor expressed mdr1, mrp1, and lrp, but not mrp2.
Neither variation of temperature, administration of the cytostatic drug, nor duration of perfusion changed the expression of this “resistance pattern”.
In contrast to the cytostatic drug, hyperthermia causes a persistent induction of HSP72.
Both observations could offer a potential explanation for failure of HILP in malignant melanoma.
American Psychological Association (APA)
Knorr, C.& Pelz, J. O.& Göhl, J.& Hohenberger, Werner& Meyer, T.. 2010. Expression of Chemoresistance-Related Genes and Heat Shock Protein 72 in Hyperthermic Isolated Limb Perfusion of Malignant Melanoma : An Experimental Study. Journal of Oncology،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-448801
Modern Language Association (MLA)
Knorr, C.…[et al.]. Expression of Chemoresistance-Related Genes and Heat Shock Protein 72 in Hyperthermic Isolated Limb Perfusion of Malignant Melanoma : An Experimental Study. Journal of Oncology No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-448801
American Medical Association (AMA)
Knorr, C.& Pelz, J. O.& Göhl, J.& Hohenberger, Werner& Meyer, T.. Expression of Chemoresistance-Related Genes and Heat Shock Protein 72 in Hyperthermic Isolated Limb Perfusion of Malignant Melanoma : An Experimental Study. Journal of Oncology. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-448801
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448801