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Investigation of a Cluster of Candida albicans Invasive Candidiasis in a Neonatal Intensive Care Unit by Pulsed-Field Gel Electrophoresis
Joint Authors
Saghrouni, Fatma
Khammari, Imene
Gheith, Soukeina
Fathallah, Akila
Boukadida, Jalel
Ben Said, Moncef
Ben Abdeljelil, Jihene
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Nosocomial invasive candidiasis (IC) has emerged as a major problem in neonatal intensive care units (NICUs).
We investigated herein the temporal clustering of six cases of neonatal IC due to Candida albicans in an NICU.
Eighteen isolates obtained from the six neonates and two isolates from two health care workers (HCWs) working at the same unit and suffering from fingers’ onychomycosis were genotyped by electrophoretic karyotyping (EK) and restriction endonuclease analysis of genomic DNA by using Sfi I (PFGE-Sfi I).
PFGE-Sfi I was more effective in discriminating between temporally related isolates.
It showed that (i) both HCWs had specific strains excluding them as a source of infections in neonates.
(ii) Isolates collected from three neonates were identical providing evidence of their clonal origin and the occurrence of a horizontal transmission of C.
albicans in the unit.
(iii) The three remaining neonates had specific strains confirming that the IC cases were coincidental.
(iv) Microevolution occurred in one catheter-related candidemia case.
Our results illustrate the relevance of the molecular approach to investigate suspected outbreaks in hospital surveys and the effectiveness of PFGE-Sfi I for typing of epidemiologically related C.
albicans isolates.
American Psychological Association (APA)
Ben Abdeljelil, Jihene& Saghrouni, Fatma& Khammari, Imene& Gheith, Soukeina& Fathallah, Akila& Ben Said, Moncef…[et al.]. 2012. Investigation of a Cluster of Candida albicans Invasive Candidiasis in a Neonatal Intensive Care Unit by Pulsed-Field Gel Electrophoresis. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-448816
Modern Language Association (MLA)
Ben Abdeljelil, Jihene…[et al.]. Investigation of a Cluster of Candida albicans Invasive Candidiasis in a Neonatal Intensive Care Unit by Pulsed-Field Gel Electrophoresis. The Scientific World Journal No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-448816
American Medical Association (AMA)
Ben Abdeljelil, Jihene& Saghrouni, Fatma& Khammari, Imene& Gheith, Soukeina& Fathallah, Akila& Ben Said, Moncef…[et al.]. Investigation of a Cluster of Candida albicans Invasive Candidiasis in a Neonatal Intensive Care Unit by Pulsed-Field Gel Electrophoresis. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-448816
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448816