A New Rapid and Sensitive Spectrophotometric Method for Determination of a Biopolymer Chitosan
Author
Source
International Journal of Carbohydrate Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A novel approach of spectrophotometric quantification of chitosan based on one-step depolymerization with sodium nitrite followed by reaction of the end product with thiobarbituric acid has been proposed, optimized, and validated.
In this process, chitosan is converted into 2,5-anhydro-D-mannose that reacts with thiobarbituric acid to form pink color.
The color that resulted from the reaction was stabilized and measured at 555 nm.
The method optimization was essential as many procedural parameters influenced the accuracy of the determination including hydrolysis conditions, thiobarbituric acid concentration, reaction time, pH, reaction temperature, and color stability period.
Under given optimized conditions that appeared to be critical, chitosan was quantitatively analyzed and the calibration graph was linear over the range of 10–50 μg/mL (r2=0.999).
This approach was applied for determination of chitosan in pharmaceutical formulation (chitocal) and had a recovery rate of higher than 96%.
The developed method is easy to use and highly accurate.
American Psychological Association (APA)
Badawy, Mohamed E. I.. 2012. A New Rapid and Sensitive Spectrophotometric Method for Determination of a Biopolymer Chitosan. International Journal of Carbohydrate Chemistry،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-448851
Modern Language Association (MLA)
Badawy, Mohamed E. I.. A New Rapid and Sensitive Spectrophotometric Method for Determination of a Biopolymer Chitosan. International Journal of Carbohydrate Chemistry No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-448851
American Medical Association (AMA)
Badawy, Mohamed E. I.. A New Rapid and Sensitive Spectrophotometric Method for Determination of a Biopolymer Chitosan. International Journal of Carbohydrate Chemistry. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-448851
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448851