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Combination of ERG9 Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene in Saccharomyces cerevisiae
Joint Authors
Prameela Devi, Yalavarthy
Parcha, Sreenivasa Rao
Baadhe, Rama Raju
Mekala, Naveen Kumar
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The yeast strain (Saccharomyces cerevisiae) MTCC 3157 was selected for combinatorial biosynthesis of plant sesquiterpene amorpha-4,11-diene.
Our main objective was to overproduce amorpha 4-11-diene, which is a key precursor molecule of artemisinin (antimalarial drug) produced naturally in plant Artemisia annua through mevalonate pathway.
Farnesyl diphosphate (FPP) is a common intermediate metabolite of a variety of compounds in the mevalonate pathway of yeast and leads to the production of ergosterols, dolichol and ubiquinone, and so forth.
In our studies, FPP converted to amorphadiene (AD) by expressing heterologous amorphadiene synthase (ADS) in yeast.
First, ERG9 (squalane synthase) promoter of yeast was replaced with repressible methionine (MET3) promoter by using bipartite gene fusion method.
Further to overcome the loss of the intermediate FPP through competitive pathways in yeast, fusion protein technology was adopted and farnesyldiphosphate synthase (FPPS) of yeast has been coupled with amorphadiene synthase (ADS) of plant origin (Artemisia annua L.) where amorphadiene production was improved by 2-fold (11.2 mg/L) and 4-fold (25.02 mg/L) in yeast strains YCF-002 and YCF-005 compared with control strain YCF-AD (5.5 mg/L), respectively.
American Psychological Association (APA)
Baadhe, Rama Raju& Mekala, Naveen Kumar& Parcha, Sreenivasa Rao& Prameela Devi, Yalavarthy. 2013. Combination of ERG9 Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene in Saccharomyces cerevisiae. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-448946
Modern Language Association (MLA)
Baadhe, Rama Raju…[et al.]. Combination of ERG9 Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene in Saccharomyces cerevisiae. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-448946
American Medical Association (AMA)
Baadhe, Rama Raju& Mekala, Naveen Kumar& Parcha, Sreenivasa Rao& Prameela Devi, Yalavarthy. Combination of ERG9 Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene in Saccharomyces cerevisiae. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-448946
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448946