Gastrointestinal Bacterial and Methanogenic Archaea Diversity Dynamics Associated with Condensed Tannin-Containing Pine Bark Diet in Goats Using 16S rDNA Amplicon Pyrosequencing
Joint Authors
Shange, Raymon
Eun, Jong-Su
Solaiman, Sandra
Min, Byeng R.
Source
International Journal of Microbiology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Eighteen Kiko-cross meat goats (n=6) were used to collect gastrointestinal (GI) bacteria and methanogenic archaea for diversity measures when fed condensed tannin-containing pine bark (PB).
Three dietary treatments were tested: control diet (0% PB and 30% wheat straw (WS); 0.17% condensed tannins (CT) dry matter (DM)); 15% PB and 15% WS (1.6% CT DM), and 30% PB and 0% WS (3.2% CT DM).
A 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing technique was used to characterize and elucidate changes in GI bacteria and methanogenic archaea diversity among the diets.
Proteobacteria was the most dominant phylum in goats with mean relative abundance values ranging from 39.7 (30% PB) to 46.5% (control) and 47.1% (15% PB).
Other phyla individually accounted for fewer than 25% of the relative abundance observed.
Predominant methanogens were Methanobrevibacter (75, 72, and 49%), Methanosphaera (3.3, 2.3, and 3.4%), and Methanobacteriaceae (1.2, 0.6, and 0.7%) population in control, 15, and 30% PB, respectively.
Among methanogens, Methanobrevibacter was linearly decreased (P=0.05) with increasing PB supplementation.
These results indicate that feeding PB selectively altered bacteria and methanogenic archaeal populations in the GI tract of goats.
American Psychological Association (APA)
Min, Byeng R.& Solaiman, Sandra& Shange, Raymon& Eun, Jong-Su. 2014. Gastrointestinal Bacterial and Methanogenic Archaea Diversity Dynamics Associated with Condensed Tannin-Containing Pine Bark Diet in Goats Using 16S rDNA Amplicon Pyrosequencing. International Journal of Microbiology،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-449060
Modern Language Association (MLA)
Min, Byeng R.…[et al.]. Gastrointestinal Bacterial and Methanogenic Archaea Diversity Dynamics Associated with Condensed Tannin-Containing Pine Bark Diet in Goats Using 16S rDNA Amplicon Pyrosequencing. International Journal of Microbiology No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-449060
American Medical Association (AMA)
Min, Byeng R.& Solaiman, Sandra& Shange, Raymon& Eun, Jong-Su. Gastrointestinal Bacterial and Methanogenic Archaea Diversity Dynamics Associated with Condensed Tannin-Containing Pine Bark Diet in Goats Using 16S rDNA Amplicon Pyrosequencing. International Journal of Microbiology. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-449060
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449060