Microbubble-Mediated Ultrasound Enhances the Lethal Effect of Gentamicin on Planktonic Escherichia coli
Joint Authors
Hu, Bin
Cai, Xun-Zi
Zhu, Han-Xiao
Shi, Zhong-Li
Yan, Shi-Gui
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Previous research has found that low-intensity ultrasound enhanced the lethal effect of gentamicin on planktonic E.
coli.
We aimed to further investigate whether microbubble-mediated low-intensity ultrasound could further enhance the antimicrobial efficacy of gentamicin.
The planktonic E.
coli (ATCC 25922) was distributed to four different interventions: control (GCON), microbubble only (GMB), ultrasound only (GUS), and microbubble-mediated ultrasound (GMUS).
Ultrasound was applied with 100 mW/cm2 (average intensity) and 46.5 KHz, which presented no bactericidal activity.
After 12 h, plate counting was used to estimate the number of bacteria, and bacterial micromorphology was observed with transmission electron microscope.
The results showed that the viable counts of E.
coli in GMUS were decreased by 1.01 to 1.42 log10 CFU/mL compared with GUS (P<0.01).
The minimal inhibitory concentration (MIC) of gentamicin against E.
coli was 1 μg/mL in the GMUS and GUS groups, lower than that in the GCON and GMB groups (2 μg/mL).
Transmission electron microscopy (TEM) images exhibited more destruction and higher thickness of bacterial cell membranes in the GMUS than those in other groups.
The reason might be the increased permeability of cell membranes for gentamicin caused by acoustic cavitation.
American Psychological Association (APA)
Zhu, Han-Xiao& Cai, Xun-Zi& Shi, Zhong-Li& Hu, Bin& Yan, Shi-Gui. 2014. Microbubble-Mediated Ultrasound Enhances the Lethal Effect of Gentamicin on Planktonic Escherichia coli. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-449084
Modern Language Association (MLA)
Zhu, Han-Xiao…[et al.]. Microbubble-Mediated Ultrasound Enhances the Lethal Effect of Gentamicin on Planktonic Escherichia coli. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-449084
American Medical Association (AMA)
Zhu, Han-Xiao& Cai, Xun-Zi& Shi, Zhong-Li& Hu, Bin& Yan, Shi-Gui. Microbubble-Mediated Ultrasound Enhances the Lethal Effect of Gentamicin on Planktonic Escherichia coli. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-449084
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449084