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Analyzing the Role of Receptor Internalization in the Regulation of Melanin-Concentrating Hormone Signaling
Joint Authors
Goodspeed, Andrew
Haude, Katrina
Moden, Jay I.
Cook, Laurie B.
Carroll, Robert
Source
International Journal of Endocrinology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The regulation of appetite is complex, though our understanding of the process is improving.
The potential role for the melanin-concentrating hormone (MCH) signaling pathway in the treatment of obesity is being explored by many.
It was hypothesized that internalization of MCH receptors would act to potently desensitize cells to MCH.
Despite potent desensitization of ERK signaling by MCH in BHK-570 cells, we were unable to observe MCH-mediated internalization of MCH receptor 1 (MCHR1) by fluorescence microscopy.
A more quantitative approach using a cell-based ELISA indicated only 15% of receptors internalized, which is much lower than that reported in the literature.
When β-arrestins were overexpressed in our system, removal of receptors from the cell surface was facilitated and signaling to a leptin promoter was diminished, suggesting that internalization of MCHR1 is sensitive to cellular β-arrestin levels.
A dominant-negative GRK construct completely inhibited loss of receptors from the cell surface in response to MCH, suggesting that the internalization observed is phosphorylation-dependent.
Since desensitization of MCH-mediated ERK signaling did not correlate with significant loss of MCHR1 from the cell surface, we hypothesize that in this model system regulation of MCH signaling may be the result of segregation of receptors from signaling components at the plasma membrane.
American Psychological Association (APA)
Moden, Jay I.& Haude, Katrina& Carroll, Robert& Goodspeed, Andrew& Cook, Laurie B.. 2013. Analyzing the Role of Receptor Internalization in the Regulation of Melanin-Concentrating Hormone Signaling. International Journal of Endocrinology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-449179
Modern Language Association (MLA)
Moden, Jay I.…[et al.]. Analyzing the Role of Receptor Internalization in the Regulation of Melanin-Concentrating Hormone Signaling. International Journal of Endocrinology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-449179
American Medical Association (AMA)
Moden, Jay I.& Haude, Katrina& Carroll, Robert& Goodspeed, Andrew& Cook, Laurie B.. Analyzing the Role of Receptor Internalization in the Regulation of Melanin-Concentrating Hormone Signaling. International Journal of Endocrinology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-449179
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449179