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Divalent Metal- and High Mobility Group N Protein-Dependent Nucleosome Stability and Conformation
Joint Authors
Ong, Michelle S.
Davey, Curt A.
Vasudevan, Dileep
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
High mobility group N proteins (HMGNs) bind specifically to the nucleosome core and act as chromatin unfolding and activating factors.
Using an all-Xenopus system, we found that HMGN1 and HMGN2 binding to nucleosomes results in distinct ion-dependent conformation and stability.
HMGN2 association with nucleosome core particle or nucleosomal array in the presence of divalent metal triggers a reversible transition to a species with much reduced electrophoretic mobility, consistent with a less compact state of the nucleosome.
Residues outside of the nucleosome binding domain are required for the activity, which is also displayed by an HMGN1 truncation product lacking part of the regulatory domain.
In addition, thermal denaturation assays show that the presence of 1 mM Mg2+> or Ca2+ gives a reduction in nucleosome core terminus stability, which is further substantially diminished by the binding of HMGN2 or truncated HMGN1.
Our findings emphasize the importance of divalent metals in nucleosome dynamics and suggest that the differential biological activities of HMGNs in chromatin activation may involve different conformational alterations and modulation of nucleosome core stability.
American Psychological Association (APA)
Ong, Michelle S.& Vasudevan, Dileep& Davey, Curt A.. 2010. Divalent Metal- and High Mobility Group N Protein-Dependent Nucleosome Stability and Conformation. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-449250
Modern Language Association (MLA)
Ong, Michelle S.…[et al.]. Divalent Metal- and High Mobility Group N Protein-Dependent Nucleosome Stability and Conformation. Journal of Nucleic Acids No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-449250
American Medical Association (AMA)
Ong, Michelle S.& Vasudevan, Dileep& Davey, Curt A.. Divalent Metal- and High Mobility Group N Protein-Dependent Nucleosome Stability and Conformation. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-449250
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449250