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A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech
Joint Authors
Hohenstein, Kristi A.
Shain, Daniel H.
Nuthulaganti, Tej
Lang, Shirley A.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Leech embryogenesis is a model for investigating cellular and molecular processes of development.
Due to the unusually large size of embryonic stem cells (teloblasts: 50–300 μm) in the glossiphoniid leech, Theromyzon tessulatum, and the presence of identifiable stem cell precursors (proteloblasts), we previously isolated a group of genes upregulated upon stem cell birth.
In the current study, we show that one of these genes, designated Theromyzon proliferation (Tpr), is required for normal stem cell genesis; specifically, transient Tpr knockdown experiments conducted with antisense oligonucleotides and monitored by semiquantitative RT-PCR, caused abnormal proteloblast proliferation leading to embryonic death, but did not overtly affect neuroectodermal or mesodermal stem cell development once these cells were born.
Tpr encodes a large glutamine-rich (∼34%) domain that shares compositional similarity with strong transcriptional enhancers many of which have been linked with trinucleotide repeat disorders (e.g., Huntington's).
American Psychological Association (APA)
Hohenstein, Kristi A.& Lang, Shirley A.& Nuthulaganti, Tej& Shain, Daniel H.. 2009. A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech. Stem Cells International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-449284
Modern Language Association (MLA)
Hohenstein, Kristi A.…[et al.]. A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech. Stem Cells International No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-449284
American Medical Association (AMA)
Hohenstein, Kristi A.& Lang, Shirley A.& Nuthulaganti, Tej& Shain, Daniel H.. A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech. Stem Cells International. 2009. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-449284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449284