PGE2 Desensitizes β-Agonist Effect on Human Lung Fibroblast-Mediated Collagen Gel Contraction through Upregulating PDE4
Joint Authors
Rennard, Stephen I.
Fang, Qiuhong
Wang, Jing
Michalski, Joel
Liu, Xiangde
Ma, Yingmin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In the current study, we investigated the effect of a long-acting β-agonist (salmeterol) and a phosphodiesterase 4 (PDE4) inhibitor (cilomilast) on human lung fibroblast-mediated collagen gel contraction.
Higher concentrations of salmeterol (10−7 and 10−6 M) inhibited fibroblast-mediated collagen gel contraction.
No effect was observed with cilomilast alone (up to 10−5 M).
In the presence of 10−8 M salmeterol, however, cilomilast could significantly inhibit fibroblast-mediated collagen gel contraction in a concentration-dependent manner (10−7~10−5 M).
Blockade of endogenous PGE2 by indomethacin further potentiated the inhibitory effect of salmeterol on fibroblast-mediated collagen gel contraction, but it did not affect cilomilast's effect.
Pretreatment with PGE2 abolished the inhibitory effect of salmeterol, but it potentiated the inhibitory effect of cilomilast on fibroblast-mediated collagen gel contraction.
Finally, indomethacin slightly inhibited PDE4C expression, while PGE2 stimulated the expression of PDE4A and -4C in human lung fibroblasts.
These findings suggest that long-acting β-agonist and PDE4 inhibitor have a synergistic effect in regulating fibroblast tissue repair functions and that PGE2 can modulate the effect of β-agonist and PDE4 inhibitor at least in part through the mechanism of regulating PDE4 expression.
American Psychological Association (APA)
Fang, Qiuhong& Ma, Yingmin& Wang, Jing& Michalski, Joel& Rennard, Stephen I.& Liu, Xiangde. 2013. PGE2 Desensitizes β-Agonist Effect on Human Lung Fibroblast-Mediated Collagen Gel Contraction through Upregulating PDE4. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-449286
Modern Language Association (MLA)
Fang, Qiuhong…[et al.]. PGE2 Desensitizes β-Agonist Effect on Human Lung Fibroblast-Mediated Collagen Gel Contraction through Upregulating PDE4. Mediators of Inflammation No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-449286
American Medical Association (AMA)
Fang, Qiuhong& Ma, Yingmin& Wang, Jing& Michalski, Joel& Rennard, Stephen I.& Liu, Xiangde. PGE2 Desensitizes β-Agonist Effect on Human Lung Fibroblast-Mediated Collagen Gel Contraction through Upregulating PDE4. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-449286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449286