Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds
Joint Authors
Gilardoni, Liliana Rosa
Jolly, Ana
Paolicchi, Fernando Alberto
Mundo, Silvia Leonor
Colavecchia, Silvia Beatriz
Fernández, Bárbara
Source
Veterinary Medicine International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-26
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Johne's Disease or Paratuberculosis is a chronic granulomatous enteritis disease affecting ruminants.
Detection of subclinically infected animals is difficult, hampering the control of this disease.
The aim of this work was to evaluate the performance of detection of IgG isotypes in a PPA-ELISA to improve the recognition of cattle naturally infected with Map in different stages.
A total of 108 animals from Tuberculosis-free herds were grouped as follows: exposed (n=30), subclinically infected (n=26), clinically infected (n=14), and healthy controls (n=38).
Receiver-operating characteristic (ROC) curves of isotypes/PPA-ELISAs were constructed and areas under the curves were compared to evaluate the performance of each test.
Our study demonstrated that the conventional PPA-ELISA (detecting IgG) is the best to identify clinically infected animals with high sensitivity (92.9%) and specificity (100%).
Meanwhile, IgG2/PPA-ELISA improved the number of subclinically infected cattle detected as compared with conventional IgG/PPA-ELISA (53.8 versus 23.1%).
In addition, it had the maximum sensitivity (65.0%, taking into account all Map-infected cattle).
In conclusion, the combination of IgG and IgG2/PPA-ELISAs may improve the identification of Map-infected cattle in different stages of disease.
The usefulness of IgG2 detection in serological tests for Johne's Disease diagnosis should be further evaluated.
American Psychological Association (APA)
Fernández, Bárbara& Gilardoni, Liliana Rosa& Jolly, Ana& Colavecchia, Silvia Beatriz& Paolicchi, Fernando Alberto& Mundo, Silvia Leonor. 2012. Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds. Veterinary Medicine International،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-449301
Modern Language Association (MLA)
Fernández, Bárbara…[et al.]. Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds. Veterinary Medicine International No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-449301
American Medical Association (AMA)
Fernández, Bárbara& Gilardoni, Liliana Rosa& Jolly, Ana& Colavecchia, Silvia Beatriz& Paolicchi, Fernando Alberto& Mundo, Silvia Leonor. Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds. Veterinary Medicine International. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-449301
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449301