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Changes of Myogenic Reactive Oxygen Species and Interleukin-6 in Contracting Skeletal Muscle Cells
Joint Authors
Chen, Yajun
Hao, Xuanming
Pan, Hongying
Xu, Xiaoyang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The aim of this study was to measure changes in myotube reactive oxygen species (ROS) and the production of interleukin (IL)-6 in electrically stimulated mouse C2C12 skeletal muscle cells.
After five days of differentiation, myotubes were stimulated using an electrical stimulator set at 45 V at a frequency of 5 Hz, with a pulse width of 20 ms.
Acute stimulations were performed for 45, 60, 75, 90, or 120 min in each dish.
ROSs were detected in the extracted cells directly using a fluorescent probe.
IL-6 mRNA expression in C2C12 myotubes and IL-6 concentration in C2C12 myotube supernatants were determined using real-time PCR and ELISA, respectively.
Compared with control cells, ROS generation was significantly increased at 45 min after the onset of stimulation (P<0.01) and continued to increase, reaching a maximum at 120 min.
IL-6 mRNA expression and IL-6 concentration in C2C12 cells were significantly increased after 75 min (P<0.01) and 120 min (P<0.05) of electrical stimulation (ES) compared with the control cells.
Our data show that a specific ES intensity may modulate ROS accumulation and affect IL-6 gene expression in contracting skeletal muscle cells.
American Psychological Association (APA)
Pan, Hongying& Xu, Xiaoyang& Hao, Xuanming& Chen, Yajun. 2012. Changes of Myogenic Reactive Oxygen Species and Interleukin-6 in Contracting Skeletal Muscle Cells. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-449310
Modern Language Association (MLA)
Pan, Hongying…[et al.]. Changes of Myogenic Reactive Oxygen Species and Interleukin-6 in Contracting Skeletal Muscle Cells. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-449310
American Medical Association (AMA)
Pan, Hongying& Xu, Xiaoyang& Hao, Xuanming& Chen, Yajun. Changes of Myogenic Reactive Oxygen Species and Interleukin-6 in Contracting Skeletal Muscle Cells. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-449310
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449310