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Identification and Characterization of Differentially Expressed Transcripts in the Gills of Freshwater Prawn (Macrobrachium rosenbergii) under Salt Stress
Joint Authors
Rath, Surya Narayan
Panda, Rudra Prasanna
Barman, Hirak Kumar
Das, Varsha
Jayasankar, Pallipuram
Mohapatra, Chinmayee
Mohanta, Ramya
Mohapatra, Shibani Dutta
Patra, Swagat Kumar
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The giant freshwater prawn, Macrobrachium rosenbergii, is an economically important species.
It is a euryhaline shrimp, surviving in wide-range salinity conditions.
A change in gene expression has been suggested as an important component for stress management.
To better understand the osmoregulatory mechanisms mediated by the gill, a subtractive and suppressive hybridization (SSH) tool was used to identify expressed transcripts linked to adaptations in saline water.
A total of 117 transcripts represented potentially expressed under salinity conditions.
BLAST analysis identified 22% as known genes, 9% as uncharacterized showing homologous to unannotated ESTs, and 69% as unknown sequences.
All the identified known genes representing broad spectrum of biological pathways were particularly linked to stress tolerance including salinity tolerance.
Expression analysis of 10 known genes and 7 unknown/uncharacterized genes suggested their upregulation in the gills of prawn exposed to saline water as compared to control indicating that these are likely to be associated with salinity acclimation.
Rapid amplification of cDNA ends (RACE) was used for obtaining full-length cDNA of MRSW-40 clone that was highly upregulated during salt exposure.
The sequenced ESTs presented here will have potential implications for future understanding about salinity acclimation and/or tolerance of the prawn.
American Psychological Association (APA)
Barman, Hirak Kumar& Patra, Swagat Kumar& Das, Varsha& Mohapatra, Shibani Dutta& Jayasankar, Pallipuram& Mohapatra, Chinmayee…[et al.]. 2012. Identification and Characterization of Differentially Expressed Transcripts in the Gills of Freshwater Prawn (Macrobrachium rosenbergii) under Salt Stress. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-449695
Modern Language Association (MLA)
Barman, Hirak Kumar…[et al.]. Identification and Characterization of Differentially Expressed Transcripts in the Gills of Freshwater Prawn (Macrobrachium rosenbergii) under Salt Stress. The Scientific World Journal No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-449695
American Medical Association (AMA)
Barman, Hirak Kumar& Patra, Swagat Kumar& Das, Varsha& Mohapatra, Shibani Dutta& Jayasankar, Pallipuram& Mohapatra, Chinmayee…[et al.]. Identification and Characterization of Differentially Expressed Transcripts in the Gills of Freshwater Prawn (Macrobrachium rosenbergii) under Salt Stress. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-449695
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449695