Antiproliferative Factor-Induced Changes in Phosphorylation and Palmitoylation of Cytoskeleton-Associated Protein-4 Regulate Its Nuclear Translocation and DNA Binding
Joint Authors
Zacharias, David A.
Planey, Sonia Lobo
Mullen, Matthew
Source
International Journal of Cell Biology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Cytoskeleton-associated protein 4 (CKAP4) is a reversibly palmitoylated and phosphorylated transmembrane protein that functions as a high-affinity receptor for antiproliferative factor (APF)—a sialoglycopeptide secreted from bladder epithelial cells of patients with interstitial cystitis (IC).
Palmitoylation of CKAP4 by the palmitoyl acyltransferase, DHHC2, is required for its cell surface localization and subsequent APF signal transduction; however, the mechanism for APF signal transduction by CKAP4 is unknown.
In this paper, we demonstrate that APF treatment induces serine phosphorylation of residues S3, S17, and S19 of CKAP4 and nuclear translocation of CKAP4.
Additionally, we demonstrate that CKAP4 binds gDNA in a phosphorylation-dependent manner in response to APF treatment, and that a phosphomimicking, constitutively nonpalmitoylated form of CKAP4 localizes to the nucleus, binds DNA, and mimics the inhibitory effects of APF on cellular proliferation.
These results reveal a novel role for CKAP4 as a downstream effecter for APF signal transduction.
American Psychological Association (APA)
Zacharias, David A.& Mullen, Matthew& Planey, Sonia Lobo. 2012. Antiproliferative Factor-Induced Changes in Phosphorylation and Palmitoylation of Cytoskeleton-Associated Protein-4 Regulate Its Nuclear Translocation and DNA Binding. International Journal of Cell Biology،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-449834
Modern Language Association (MLA)
Zacharias, David A.…[et al.]. Antiproliferative Factor-Induced Changes in Phosphorylation and Palmitoylation of Cytoskeleton-Associated Protein-4 Regulate Its Nuclear Translocation and DNA Binding. International Journal of Cell Biology No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-449834
American Medical Association (AMA)
Zacharias, David A.& Mullen, Matthew& Planey, Sonia Lobo. Antiproliferative Factor-Induced Changes in Phosphorylation and Palmitoylation of Cytoskeleton-Associated Protein-4 Regulate Its Nuclear Translocation and DNA Binding. International Journal of Cell Biology. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-449834
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449834