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Comparison of Two Methods of RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissue Specimens
Joint Authors
Ferreira, Suzete Cleusa
Pereira, Juliana
Ferreira, Jerenice Esdras
Gouveia, Gisele Rodrigues
Siqueira, Sheila Aparecida Coelho
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-03
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The present study aimed to compare two different methods of extracting RNA from formalin-fixed paraffin-embedded (FFPE) specimens of diffuse large B-cell lymphoma (DLBCL).
We further aimed to identify possible influences of variables—such as tissue size, duration of paraffin block storage, fixative type, primers used for cDNA synthesis, and endogenous genes tested—on the success of amplification from the samples.
Both tested protocols used the same commercial kit for RNA extraction (the RecoverAll Total Nucleic Acid Isolation Optimized for FFPE Samples from Ambion).
However, the second protocol included an additional step of washing with saline buffer just after sample rehydration.
Following each protocol, we compared the RNA amount and purity and the amplification success as evaluated by standard PCR and real-time PCR.
The results revealed that the extra washing step added to the RNA extraction process resulted in significantly improved RNA quantity and quality and improved success of amplification from paraffin-embedded specimens.
American Psychological Association (APA)
Gouveia, Gisele Rodrigues& Ferreira, Suzete Cleusa& Ferreira, Jerenice Esdras& Siqueira, Sheila Aparecida Coelho& Pereira, Juliana. 2014. Comparison of Two Methods of RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissue Specimens. BioMed Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-449903
Modern Language Association (MLA)
Gouveia, Gisele Rodrigues…[et al.]. Comparison of Two Methods of RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissue Specimens. BioMed Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-449903
American Medical Association (AMA)
Gouveia, Gisele Rodrigues& Ferreira, Suzete Cleusa& Ferreira, Jerenice Esdras& Siqueira, Sheila Aparecida Coelho& Pereira, Juliana. Comparison of Two Methods of RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissue Specimens. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-449903
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449903