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Changes in the Distribution of the α3 Na+K+ ATPase Subunit in Heterozygous Lurcher Purkinje Cells as a Genetic Model of Chronic Depolarization during Development
Joint Authors
McFarland, Rebecca
Vogel, Michael W.
Mariani, Jean
Zanjani, Hadi S.
Source
International Journal of Cell Biology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A common assumption of excitotoxic mechanisms in the nervous system is that the ionic imbalance resulting from overstimulation of glutamate receptors and increased Na+ and Ca++ influx overwhelms cellular energy metabolic systems leading to cell death.
The goal of this study was to examine how a chronic Na+ channel leak current in developing Purkinje cells in the heterozygous Lurcher mutant (+/Lc) affects the expression and distribution of the α3 subunit of the Na+/K+ ATPase pump, a key component of the homeostasis system that maintains ionic equilibrium in neurons.
The expression pattern of the catalytic α3 Na+/K+ ATPase subunit was analyzed by immunohistochemistry, histochemistry, and Western Blots in wild type (WT) and +/Lc cerebella at postnatal days P10, P15, and P25 to determine if there are changes in the distribution of active Na+/K+ ATPase subunits in degenerating Purkinje cells.
The results suggest that the expression of the catalytic α3 subunit is altered in chronically depolarized +/Lc Purkinje cells, although the density of active Na+/K+ ATPase pumps is not significantly altered compared with WT in the cerebellar cortex at P15, and then declines from P15 to P25 in the +/Lc cerebellum as the +/Lc Purkinje cells degenerate.
American Psychological Association (APA)
McFarland, Rebecca& Zanjani, Hadi S.& Mariani, Jean& Vogel, Michael W.. 2014. Changes in the Distribution of the α3 Na+K+ ATPase Subunit in Heterozygous Lurcher Purkinje Cells as a Genetic Model of Chronic Depolarization during Development. International Journal of Cell Biology،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-449976
Modern Language Association (MLA)
McFarland, Rebecca…[et al.]. Changes in the Distribution of the α3 Na+K+ ATPase Subunit in Heterozygous Lurcher Purkinje Cells as a Genetic Model of Chronic Depolarization during Development. International Journal of Cell Biology No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-449976
American Medical Association (AMA)
McFarland, Rebecca& Zanjani, Hadi S.& Mariani, Jean& Vogel, Michael W.. Changes in the Distribution of the α3 Na+K+ ATPase Subunit in Heterozygous Lurcher Purkinje Cells as a Genetic Model of Chronic Depolarization during Development. International Journal of Cell Biology. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-449976
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449976