Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer
Joint Authors
Oliani, Sonia Maria
Mataruco, Mayra Mioto
Rahal, Paula
Rossi, Ana Flávia Teixeira
Valsechi, Marina Curado
Fazzio, Célia Sebastiana de Jesus
Araújo, Leandro Pires
de Oliveira, Juliana Garcia
Jorge, Yvana Cristina
Silva, Ana Elizabete
Miyazaki, Kenji
Caetano, Alaor
Thomé, Jorge Alberto
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression.
This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H.
pylori infection.
Methods.
We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay.
Results.
High ANXA1 mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ = 4.26 ± 2.03) and in 80% (16/20) of GA cases (mean RQ = 4.38 ± 4.77).
However, LGALS1 mRNA levels were high (mean RQ = 2.44 ± 3.26) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ = 0.43±3.13; P<0.01).
Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins.
Conclusion.
These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis.
American Psychological Association (APA)
Jorge, Yvana Cristina& Mataruco, Mayra Mioto& Araújo, Leandro Pires& Rossi, Ana Flávia Teixeira& de Oliveira, Juliana Garcia& Valsechi, Marina Curado…[et al.]. 2013. Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-450000
Modern Language Association (MLA)
Jorge, Yvana Cristina…[et al.]. Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer. Mediators of Inflammation No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-450000
American Medical Association (AMA)
Jorge, Yvana Cristina& Mataruco, Mayra Mioto& Araújo, Leandro Pires& Rossi, Ana Flávia Teixeira& de Oliveira, Juliana Garcia& Valsechi, Marina Curado…[et al.]. Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-450000
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450000