Transient Silencing of a Type IV P-Type ATPase, Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes
Joint Authors
Dhar, M. S.
Biggerstaff, J.
Hurst, S. E.
Minkin, S. C.
Source
Journal of Nutrition and Metabolism
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Atp10c is a strong candidate gene for diet-induced obesity and type 2 diabetes.
To identify molecular and cellular targets of ATP10C, Atp10c expression was altered in vitro in C2C12 skeletal muscle myotubes by transient transfection with an Atp10c-specific siRNA.
Glucose uptake assays revealed that insulin stimulation caused a significant 2.54-fold decrease in 2-deoxyglucose uptake in transfected cells coupled with a significant upregulation of native mitogen-activated protein kinases (MAPKs), p38, and p44/42.
Additionally, glucose transporter-1 (GLUT1) was significantly upregulated; no changes in glucose transporter-4 (GLUT4) expression were observed.
The involvement of MAPKs was confirmed using the specific inhibitor SB203580, which downregulated the expression of native and phosphorylated MAPK proteins in transfected cells without any changes in insulin-stimulated glucose uptake.
Results indicate that Atp10c regulates glucose metabolism, at least in part via the MAPK pathway, and, thus, plays a significant role in the development of insulin resistance and type 2 diabetes.
American Psychological Association (APA)
Hurst, S. E.& Minkin, S. C.& Biggerstaff, J.& Dhar, M. S.. 2012. Transient Silencing of a Type IV P-Type ATPase, Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes. Journal of Nutrition and Metabolism،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-450004
Modern Language Association (MLA)
Hurst, S. E.…[et al.]. Transient Silencing of a Type IV P-Type ATPase, Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes. Journal of Nutrition and Metabolism No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-450004
American Medical Association (AMA)
Hurst, S. E.& Minkin, S. C.& Biggerstaff, J.& Dhar, M. S.. Transient Silencing of a Type IV P-Type ATPase, Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes. Journal of Nutrition and Metabolism. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-450004
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450004