An Assessment of Epithelial and Mesenchymal Phenotypes in Experimental and Clinical Pulmonary Fibrosis
Joint Authors
Zhang, Huilan
Herzog, Erica L.
Carruthers, Alan M.
Murray, Lynne A.
Knight, Darryl A.
Bell, Matthew J.
Martinez, Fernando J.
Sleeman, Matthew A.
Peng, Xueyang
Hogaboam, Cory M.
Dunmore, Rebecca
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Epithelial injury has been implicated as a driving factor for the pathogenesis of idiopathic pulmonary fibrosis (IPF).
In this study we investigated changes in epithelial and mesenchymal markers in experimental models of fibrosis and associated this with IPF.
TGFβ1 induced an epithelial to mesenchymal transition (EMT) phenotype in A549 cells and normal human bronchial epithelial cells, with A549 cells exhibiting a more profound transition to a mesenchymal phenotype.
TGFβ1 overexpression in the lungs of mice resulted in an early increase in mesenchymal cell markers and apoptotic genes that preceded collagen deposition, suggesting an early epithelial injury triggers the downstream fibrotic response.
In contrast, bleomycin had a gradual increase in mesenchymal cell marker and a decrease in E-cadherin expression that correlated with collagen protein deposition.
Finally, we compared normal healthy lung tissue with surgical lung biopsies from IPF patients and observed alterations in epithelial and mesenchymal cell markers, as well as an increase in the apoptotic marker GSK3β.
Interestingly, the mesenchymal changes were more profound in rapidly progressive patients in comparison to IPF patients with slowing progressing disease.
In summary, this study provides evidence of alterations in epithelial and mesenchymal markers in experimental models of lung fibrosis and how these findings are relevant to clinical disease.
American Psychological Association (APA)
Dunmore, Rebecca& Carruthers, Alan M.& Bell, Matthew J.& Zhang, Huilan& Hogaboam, Cory M.& Peng, Xueyang…[et al.]. 2012. An Assessment of Epithelial and Mesenchymal Phenotypes in Experimental and Clinical Pulmonary Fibrosis. ISRN Pulmonology،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-450104
Modern Language Association (MLA)
Dunmore, Rebecca…[et al.]. An Assessment of Epithelial and Mesenchymal Phenotypes in Experimental and Clinical Pulmonary Fibrosis. ISRN Pulmonology No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-450104
American Medical Association (AMA)
Dunmore, Rebecca& Carruthers, Alan M.& Bell, Matthew J.& Zhang, Huilan& Hogaboam, Cory M.& Peng, Xueyang…[et al.]. An Assessment of Epithelial and Mesenchymal Phenotypes in Experimental and Clinical Pulmonary Fibrosis. ISRN Pulmonology. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-450104
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450104