PINK1-Interacting Proteins : Proteomic Analysis of Overexpressed PINK1
Joint Authors
Klein, Christine
Grünewald, Anne
Lohmann, Katja
Rakovic, Aleksandar
Hofmann, Sarah
Voges, Lisa
Orolicki, Slobodanka
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Recent publications suggest that the Parkinson's disease- (PD-) related PINK1/Parkin pathway promotes elimination of dysfunctional mitochondria by autophagy.
We used tandem affinity purification (TAP), SDS-PAGE, and mass spectrometry as a first step towards identification of possible substrates for PINK1.
The cellular abundance of selected identified interactors was investigated by Western blotting.
Furthermore, one candidate gene was sequenced in 46 patients with atypical PD.
In addition to two known binding partners (HSP90, CDC37), 12 proteins were identified using the TAP assay; four of which are mitochondrially localized (GRP75, HSP60, LRPPRC, and TUFM).
Western blot analysis showed no differences in cellular abundance of these proteins comparing PINK1 mutant and control fibroblasts.
When sequencing LRPPRC, four exonic synonymous changes and 20 polymorphisms in noncoding regions were detected.
Our study provides a list of putative PINK1 binding partners, confirming previously described interactions, but also introducing novel mitochondrial proteins as potential components of the PINK1/Parkin mitophagy pathway.
American Psychological Association (APA)
Rakovic, Aleksandar& Grünewald, Anne& Voges, Lisa& Hofmann, Sarah& Orolicki, Slobodanka& Lohmann, Katja…[et al.]. 2011. PINK1-Interacting Proteins : Proteomic Analysis of Overexpressed PINK1. Psyche،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-450108
Modern Language Association (MLA)
Rakovic, Aleksandar…[et al.]. PINK1-Interacting Proteins : Proteomic Analysis of Overexpressed PINK1. Psyche No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-450108
American Medical Association (AMA)
Rakovic, Aleksandar& Grünewald, Anne& Voges, Lisa& Hofmann, Sarah& Orolicki, Slobodanka& Lohmann, Katja…[et al.]. PINK1-Interacting Proteins : Proteomic Analysis of Overexpressed PINK1. Psyche. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-450108
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450108