miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing
Joint Authors
Zheng, Ping
Ji, Weizhi
Tan, Tao
Zhang, Yanfeng
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Spermatogonial stem cells (SSCs) play fundamental roles in spermatogenesis.
Although a handful of genes have been discovered as key regulators of SSC self-renewal and differentiation, the regulatory network responsible for SSC function remains unclear.
In particular, small RNA signatures during mouse spermatogenesis are not yet systematically investigated.
Here, using next generation sequencing, we compared small RNA signatures of in vitro expanded SSCs, testis-derived somatic cells (Sertoli cells), developing germ cells, mouse embryonic stem cells (ESCs), and mouse mesenchymal stem cells among mouse embryonic stem cells (ESCs) to address small RNA transition during mouse spermatogenesis.
The results manifest that small RNA transition during mouse spermatogenesis displays overall declined expression profiles of miRNAs and endo-siRNAs, in parallel with elevated expression profiles of piRNAs, resulting in the normal biogenesis of sperms.
Meanwhile, several novel miRNAs were preferentially expressed in mouse SSCs, and further investigation of their functional annotation will allow insights into the mechanisms involved in the regulation of SSC activities.
We also demonstrated the similarity of miRNA signatures between SSCs and ESCs, thereby providing a new clue to understanding the molecular basis underlying the easy conversion of SSCs to ESCs.
American Psychological Association (APA)
Tan, Tao& Zhang, Yanfeng& Ji, Weizhi& Zheng, Ping. 2014. miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-450138
Modern Language Association (MLA)
Tan, Tao…[et al.]. miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-450138
American Medical Association (AMA)
Tan, Tao& Zhang, Yanfeng& Ji, Weizhi& Zheng, Ping. miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-450138
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450138