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Cationic Bioactive Peptide from the Seeds of Benincasa hispida
Joint Authors
Verma, Hirday Narain
Sharma, Sunayana
Sharma, Nilesh Kumar
Source
International Journal of Peptides
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A designated bioactive peptide “Hispidalin” purified from the seeds of Benincasa hispida, which is a medicinal plant, belongs to Cucurbitaceae family.
Purification was achieved by using a procedure consisting of extraction from potassium phosphate buffer followed by FPLC and HPLC steps.
Based on amino acid residue, this peptide is amphipathic and basic with one net positive charge having isoelectric pH 8.1.
This peptide is without sulphur containing amino acid suggesting its extended conformation lacking double bond secondary structure.
The results obtained from MALDI-TOF suggested that Hispidalin is of molecular mass 5.7 KDa with 49 amino acid residues and confirmed SDS-PAGE resolved ∼6.0 KDa protein band.
This novel and unknown peptide “Hispidalin” showed broad and potent inhibitory effects against various human bacterial and fungal pathogens; its growth inhibition was significantly comparable with commercial antibacterial and antifungal drugs.
The Hispidalin at 40 μg/mL concentration exhibited 70.8% DPPH free radical-scavenging activity and 69.5% lipid peroxide inhibition.
Thus, in the present study, Hispidalin demonstrated remarkable antimicrobial and antioxidant potentials from the seeds of B.
hispida.
American Psychological Association (APA)
Sharma, Sunayana& Verma, Hirday Narain& Sharma, Nilesh Kumar. 2014. Cationic Bioactive Peptide from the Seeds of Benincasa hispida. International Journal of Peptides،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-450232
Modern Language Association (MLA)
Sharma, Sunayana…[et al.]. Cationic Bioactive Peptide from the Seeds of Benincasa hispida. International Journal of Peptides No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-450232
American Medical Association (AMA)
Sharma, Sunayana& Verma, Hirday Narain& Sharma, Nilesh Kumar. Cationic Bioactive Peptide from the Seeds of Benincasa hispida. International Journal of Peptides. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-450232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450232