Durable Expression of Minicircle DNA-Liposome-Delivered Androgen Receptor cDNA in Mice with Hepatocellular Carcinoma
Joint Authors
Chuang, Wei-Min
Jeng, Long-Bin
Chang, Tian-You
Chung, Chin-Ying
Li, Long-Yuan
Ma, Wen-Lung
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
The most common gene-based cancer therapies involve the suppression of oncogenic molecules and enhancement of the expression of tumor-suppressor genes.
Studies in noncancer disease animal models have shown that minicircle (MC) DNA vectors are easy to deliver and that the proteins from said MC-carrying DNA vectors are expressed over a long period of time.
However, delivery of therapeutic genes via a liposome-mediated, MC DNA complex has never been tested in vascular-rich hepatocellular carcinoma (HCC).
Liposome-mediated DNA delivery exhibits high in vivo transfection efficiency and minimal systemic immune response, thereby allowing for repetitive interventions.
In this study, we evaluated the efficacy of delivering an MC-liposome vector containing a 3.2 kb androgen receptor (AR; HCC metastasis suppressor) cDNA into Hepatitis B Virus- (HBV-) induced HCC mouse livers.
Results.
Protein expression and promoter luciferase assays revealed that liposome-encapsulated MC-AR resulted in abundant functional expression of AR protein (100 kD) for up to two weeks.
The AR cDNA was also successfully delivered into normal livers and diseased livers, where it was persistently expressed.
In both normal livers and livers with tumors, the expression of AR was detectable for up to 60 days.
Conclusion.
Our results show that an MC/liposome delivery system might improve the efficacy of gene therapy in patients with HCC.
American Psychological Association (APA)
Chang, Tian-You& Chung, Chin-Ying& Chuang, Wei-Min& Li, Long-Yuan& Jeng, Long-Bin& Ma, Wen-Lung. 2014. Durable Expression of Minicircle DNA-Liposome-Delivered Androgen Receptor cDNA in Mice with Hepatocellular Carcinoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-450266
Modern Language Association (MLA)
Chang, Tian-You…[et al.]. Durable Expression of Minicircle DNA-Liposome-Delivered Androgen Receptor cDNA in Mice with Hepatocellular Carcinoma. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-450266
American Medical Association (AMA)
Chang, Tian-You& Chung, Chin-Ying& Chuang, Wei-Min& Li, Long-Yuan& Jeng, Long-Bin& Ma, Wen-Lung. Durable Expression of Minicircle DNA-Liposome-Delivered Androgen Receptor cDNA in Mice with Hepatocellular Carcinoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-450266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450266