Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries
Joint Authors
Kasahara, Yuuya
Kuwahara, Masayasu
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Specific binders comprised of nucleic acids, that is, RNA/DNA aptamers, are attractive functional biopolymers owing to their potential broad application in medicine, food hygiene, environmental analysis, and biological research.
Despite the large number of reports on selection of natural DNA/RNA aptamers, there are not many examples of direct screening of chemically modified nucleic acid aptamers.
This is because of (i) the inferior efficiency and accuracy of polymerase reactions involving transcription/reverse-transcription of modified nucleotides compared with those of natural nucleotides, (ii) technical difficulties and additional time and effort required when using modified nucleic acid libraries, and (iii) ambiguous efficacies of chemical modifications in binding properties until recently; in contrast, the effects of chemical modifications on biostability are well studied using various nucleotide analogs.
Although reports on the direct screening of a modified nucleic acid library remain in the minority, chemical modifications would be essential when further functional expansion of nucleic acid aptamers, in particular for medical and biological uses, is considered.
This paper focuses on enzymatic production of chemically modified nucleic acids and their application to random screenings.
In addition, recent advances and possible future research are also described.
American Psychological Association (APA)
Kasahara, Yuuya& Kuwahara, Masayasu. 2012. Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-450279
Modern Language Association (MLA)
Kasahara, Yuuya& Kuwahara, Masayasu. Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries. Journal of Nucleic Acids No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-450279
American Medical Association (AMA)
Kasahara, Yuuya& Kuwahara, Masayasu. Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-450279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450279