Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms
Joint Authors
Seldin, Lucy
Korenblum, Elisa
Penna, Monica
Souza, Diogo Bastos
Source
International Journal of Microbiology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Crude oil samples with high- and low-water content from two offshore platforms (PA and PB) in Campos Basin, Brazil, were assessed for bacterial communities by 16S rRNA gene-based clone libraries.
RDP Classifier was used to analyze a total of 156 clones within four libraries obtained from two platforms.
The clone sequences were mainly affiliated with Gammaproteobacteria (78.2% of the total clones); however, clones associated with Betaproteobacteria (10.9%), Alphaproteobacteria (9%), and Firmicutes (1.9%) were also identified.
Pseudomonadaceae was the most common family affiliated with these clone sequences.
The sequences were further analyzed by MOTHUR, yielding 81 operational taxonomic units (OTUs) grouped at 97% stringency.
Richness estimators also calculated by MOTHUR indicated that oil samples with high-water content were the most diverse.
Comparison of bacterial communities present in these four samples using LIBSHUFF and Principal Component Analysis (PCA) indicated that the water content significantly influenced the community structure only of crude oil obtained from PA.
Differences between PA and PB libraries were observed, suggesting the importance of the oil field as a driver of community composition in this habitat.
American Psychological Association (APA)
Korenblum, Elisa& Souza, Diogo Bastos& Penna, Monica& Seldin, Lucy. 2012. Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms. International Journal of Microbiology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-450284
Modern Language Association (MLA)
Korenblum, Elisa…[et al.]. Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms. International Journal of Microbiology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-450284
American Medical Association (AMA)
Korenblum, Elisa& Souza, Diogo Bastos& Penna, Monica& Seldin, Lucy. Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms. International Journal of Microbiology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-450284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450284