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Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers) : Intervention in the Bcl-2-Mediated Apoptotic Pathway
Joint Authors
Mohamed Elhassan Taha, Manal
Alkharfy, Khalid M.
Abdel Wahab, Siddig Ibrahim
Mariod, Abdalbasit Adam
Mohan, Syam
Abdul, Ahmad Bustamam
Abdulla, Mahmood Ameen
al-Mekhlafi, Nabil
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Antiapoptotic and antioxidant activities of aqueous-methanolic extract (CAME) of Orthosiphonstamineus Benth(OS), and its hexane (HF), chloroform (CF), n-butanol (NBF), ethyl acetate (EAF) and water (WF) fractions were investigated.
Antioxidant properties were evaluated using the assays of Folin-Ciocalteu, aluminiumtrichloride, β-carotene bleaching and DPPH.
The role of OS against hydrogen peroxide induced apoptosis on MDA-M231 epithelial cells was examined using MTT assay, phase contrast microscope, colorimetric assay of caspase-3, western blot and quantitative real-time PCR.
Results showed that EAF showed the highest total phenolic content followed by CAME, NBF, WF, CF and HF, respectively.
Flavonoid content was in the order of the CF > EAF > HF > CAME > NBF > WF.
The IC50 values on DPPH assay for different extract/fractions were 126.2 ± 23, 31.25 ± 1.2, 15.25 ± 2.3, 13.56 ± 1.9, 23.0 ± 3.2, and 16.66 ± 1.5 μg/ml for HF, CF, EAF, NBF, WF and CAME, respectively.
OSreduced the oxidation of β-carotene by hydroperoxides.
Cell death was dose-dependently inhibited by pretreatment with OS.
Caspase-3 and distinct morphological features suggest the anti-apoptotic activities of OS.
This plant not only increased the expression of Bcl-2, but also decreased Bax expression, and ultimately reduced H2O2-induced apoptosis.
The current results showed that phenolics may provide health and nutritional benefits.
American Psychological Association (APA)
Abdel Wahab, Siddig Ibrahim& Mohan, Syam& Mohamed Elhassan Taha, Manal& al-Mekhlafi, Nabil& Mariod, Abdalbasit Adam& Abdul, Ahmad Bustamam…[et al.]. 2010. Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers) : Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-450298
Modern Language Association (MLA)
Abdel Wahab, Siddig Ibrahim…[et al.]. Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers) : Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-450298
American Medical Association (AMA)
Abdel Wahab, Siddig Ibrahim& Mohan, Syam& Mohamed Elhassan Taha, Manal& al-Mekhlafi, Nabil& Mariod, Abdalbasit Adam& Abdul, Ahmad Bustamam…[et al.]. Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers) : Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine. 2010. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-450298
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450298