The Effects of Apigenin on the Expression of FasFasL Apoptotic Pathway in Warm Liver Ischemia-Reperfusion Injury in Rats
Joint Authors
Papachristou, Fotini
Chatzaki, Ekaterini
Pitiakoudis, Michael
Tsaroucha, Alexandra K.
Simopoulos, Konstantinos
Tsalkidou, Evanthia G.
Tripsianis, Grigorios
Vaos, Georgios
Lambropoulou, Maria
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
The aim of this experimental study was to investigate the role of apigenin in liver apoptosis, in an experimental model of hepatic ischemia-reperfusion in rats.
Materials and Methods.
Forty-eight Wistar rats (apigenin and control groups), 14 to 16 weeks old and weighing 220 to 350 g, were used.
They were all subjected to hepatic ischemia by occlusion of the hepatic artery and portal vein for 45 minutes and reperfusion was followed for 60, 120, and 240 minutes.
Apigenin was administrated intraperitoneally.
Liver tissues were used for the detection of apoptosis by TUNEL assay and caspase 3 antibodies.
Expression analysis of Fas/FasL genes was evaluated by real time PCR.
Results.
The expression analysis of Fas and FasL genes was increasing during reperfusion (significantly in the group of 240 minutes of reperfusion).
It was in the same group that apigenin decreased Fas receptor levels and inhibited apoptosis as confirmed by TUNEL assay and caspase 3 antibodies.
Conclusions.
The effects of apigenin in the Fas/FasL mediated pathway of apoptosis, in the hepatic ischemia-reperfusion, seem to have a protective result on the hepatic cell.
American Psychological Association (APA)
Tsalkidou, Evanthia G.& Tsaroucha, Alexandra K.& Chatzaki, Ekaterini& Lambropoulou, Maria& Papachristou, Fotini& Tripsianis, Grigorios…[et al.]. 2014. The Effects of Apigenin on the Expression of FasFasL Apoptotic Pathway in Warm Liver Ischemia-Reperfusion Injury in Rats. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-450361
Modern Language Association (MLA)
Tsalkidou, Evanthia G.…[et al.]. The Effects of Apigenin on the Expression of FasFasL Apoptotic Pathway in Warm Liver Ischemia-Reperfusion Injury in Rats. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-450361
American Medical Association (AMA)
Tsalkidou, Evanthia G.& Tsaroucha, Alexandra K.& Chatzaki, Ekaterini& Lambropoulou, Maria& Papachristou, Fotini& Tripsianis, Grigorios…[et al.]. The Effects of Apigenin on the Expression of FasFasL Apoptotic Pathway in Warm Liver Ischemia-Reperfusion Injury in Rats. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-450361
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450361