![](/images/graphics-bg.png)
Biochemical and Structural Characterization of Amy1 : An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae
Joint Authors
Moraes, Lídia Maria Pepe de
Álvares, Alice Cunha Morales
Lottermann, Muriele Taborda
Torres, Fernando Araripe Gonçalves
Silva, Roberto Nascimento
Galdino, Alexsandro Sobreira
Freitas, Sonia Maria de
Ulhoa, Cirano José
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
An extracellular alpha-amylase (Amy1) whose gene from Cryptococcus flavus was previously expressed in Saccharomyces cerevisiae was purified to homogeneity (67 kDa) by ion-exchange and molecular exclusion chromatography.
The enzyme was activated by NH4+ and inhibited by Cu+2 and Hg+2.
Significant biochemical and structural discrepancies between wild-type and recombinant α-amylase with respect to Km values, enzyme specificity, and secondary structure content were found.
Far-UV CD spectra analysis at pH 7.0 revealed the high thermal stability of both proteins and the difference in folding pattern of Amy1 compared with wild-type amylase from C.
flavus, which reflected in decrease (10-fold) of enzymatic activity of recombinant protein.
Despite the differences, the highest activity of Amy1 towards soluble starch, amylopectin, and amylase, in contrast with the lowest activity of Amy1w, points to this protein as being of paramount biotechnological importance with many applications ranging from food industry to the production of biofuels.
American Psychological Association (APA)
Galdino, Alexsandro Sobreira& Silva, Roberto Nascimento& Lottermann, Muriele Taborda& Álvares, Alice Cunha Morales& Moraes, Lídia Maria Pepe de& Torres, Fernando Araripe Gonçalves…[et al.]. 2011. Biochemical and Structural Characterization of Amy1 : An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae. Enzyme Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-450370
Modern Language Association (MLA)
Galdino, Alexsandro Sobreira…[et al.]. Biochemical and Structural Characterization of Amy1 : An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae. Enzyme Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-450370
American Medical Association (AMA)
Galdino, Alexsandro Sobreira& Silva, Roberto Nascimento& Lottermann, Muriele Taborda& Álvares, Alice Cunha Morales& Moraes, Lídia Maria Pepe de& Torres, Fernando Araripe Gonçalves…[et al.]. Biochemical and Structural Characterization of Amy1 : An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-450370
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450370