Behavioral Characterization of GCLM-Knockout Mice, a Model for Enhanced Susceptibility to Oxidative Stress
Joint Authors
Kavanagh, Terrance J.
Mohar, Isaac
Cole, Toby B.
Co, Aila L.
Costa, Lucio Guido
Giordano, Gennaro
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Pharmacy, Health & Medical Sciences
Medicine
Abstract EN
Glutathione (GSH) is a major player in cellular defense against oxidative stress.
Deletion of the modifier subunit of glutamate cysteine ligase (GCLM), the first and the rate-limiting enzyme in the synthesis of GSH, leads to significantly lower GSH levels in all tissues including the brain.
GCLM-knockout (Gclm−/−) mice may thus represent a model for compromised response to oxidative stress amenable to in vitro and in vivo investigations.
In order to determine whether the diminished GSH content would by itself cause behavioral alterations, a series of behavioral tests were carried out comparing young adult Gclm−/− with wild-type mice.
Tests included the rotarod, acoustic startle reflex and prepulse inhibition of the startle reflex, open field behavior, and the platform reversal variant of the Morris Water Maze.
Results showed no differences between Gclm−/− and wild-type mice in any of the neurobehavioral tests.
However, more subtle alterations, or changes which may appear as animals age, cannot be excluded.
American Psychological Association (APA)
Cole, Toby B.& Giordano, Gennaro& Co, Aila L.& Mohar, Isaac& Kavanagh, Terrance J.& Costa, Lucio Guido. 2011. Behavioral Characterization of GCLM-Knockout Mice, a Model for Enhanced Susceptibility to Oxidative Stress. Journal of Toxicology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-450409
Modern Language Association (MLA)
Cole, Toby B.…[et al.]. Behavioral Characterization of GCLM-Knockout Mice, a Model for Enhanced Susceptibility to Oxidative Stress. Journal of Toxicology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-450409
American Medical Association (AMA)
Cole, Toby B.& Giordano, Gennaro& Co, Aila L.& Mohar, Isaac& Kavanagh, Terrance J.& Costa, Lucio Guido. Behavioral Characterization of GCLM-Knockout Mice, a Model for Enhanced Susceptibility to Oxidative Stress. Journal of Toxicology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-450409
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450409