Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining
Joint Authors
Lin, Jiapeng
Wang, Liqin
Wang, Jing
Huang, Juncheng
Zhao, Yuncheng
Chen, Tong
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects.
Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies.
Generally, determination of oocyte quality is mostly based on the layers of cumulus cells and cytoplasm or cytoplasm uniformity and colors.
This requires considerable experience to better identify oocyte quality because of the intense subjectivity involved (Gordon (2003), Madison et al.
(1992) and De Loos et al.
(1992)).
BCB staining is a function of glucose-6-phosphate dehydrogenase (G6PD) activity, an enzyme synthesized in developing oocytes, which decreases in activity with maturation.
Therefore, unstained oocytes (BCB−) are high in G6PD activity, while the less mature oocytes stains are deep blue (BCB+) due to insuffcient G6PD activity to decolorize the BCB dye.
American Psychological Association (APA)
Wang, Liqin& Lin, Jiapeng& Huang, Juncheng& Wang, Jing& Zhao, Yuncheng& Chen, Tong. 2012. Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-450734
Modern Language Association (MLA)
Wang, Liqin…[et al.]. Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-450734
American Medical Association (AMA)
Wang, Liqin& Lin, Jiapeng& Huang, Juncheng& Wang, Jing& Zhao, Yuncheng& Chen, Tong. Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-450734
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450734