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The Role of Microclot Formation in an Acute Subarachnoid Hemorrhage Model in the Rabbit
Joint Authors
Andereggen, Lukas
Widmer, Hans Rudolf
Fandino, Javier
Neuschmelting, Volker
von Gunten, Michael
Marbacher, Serge
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Microvascular dysfunction and microthrombi formation are believed to contribute to development of early brain injury (EBI) after aneurysmal subarachnoid hemorrhage (SAH).
Objective.
This study aimed to determine (i) extent of microthrombus formation and neuronal apoptosis in the brain parenchyma using a blood shunt SAH model in rabbits; (ii) correlation of structural changes in microvessels with EBI characteristics.
Methods.
Acute SAH was induced using a rabbit shunt cisterna magna model.
Extent of microthrombosis was detected 24 h post-SAH (n=8) by fibrinogen immunostaining, compared to controls (n=4).
We assessed apoptosis by terminal deoxynucleotidyl transferase nick end labeling (TUNEL) in cortex and hippocampus.
Results.
Our results showed significantly more TUNEL-positive cells (SAH: 115 ± 13; controls: 58 ± 10; P=0.016) and fibrinogen-positive microthromboemboli (SAH: 9 ± 2; controls: 2 ± 1; P=0.03) in the hippocampus after aneurysmal SAH.
Conclusions.
We found clear evidence of early microclot formation in a rabbit model of acute SAH.
The extent of microthrombosis did not correlate with early apoptosis or CPP depletion after SAH; however, the total number of TUNEL positive cells in the cortex and the hippocampus significantly correlated with mean CPP reduction during the phase of maximum depletion after SAH induction.
Both microthrombosis and neuronal apoptosis may contribute to EBI and subsequent DCI.
American Psychological Association (APA)
Andereggen, Lukas& Neuschmelting, Volker& von Gunten, Michael& Widmer, Hans Rudolf& Fandino, Javier& Marbacher, Serge. 2014. The Role of Microclot Formation in an Acute Subarachnoid Hemorrhage Model in the Rabbit. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-450760
Modern Language Association (MLA)
Andereggen, Lukas…[et al.]. The Role of Microclot Formation in an Acute Subarachnoid Hemorrhage Model in the Rabbit. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-450760
American Medical Association (AMA)
Andereggen, Lukas& Neuschmelting, Volker& von Gunten, Michael& Widmer, Hans Rudolf& Fandino, Javier& Marbacher, Serge. The Role of Microclot Formation in an Acute Subarachnoid Hemorrhage Model in the Rabbit. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-450760
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450760